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View Article and Find Full Text PDFA previous study had established that anaerobic continuous-flow (CF) cultures of conventional mouse cecal flora were able to maintain the in vivo ecological balance among the indigenous bacterial species tested. This paper describes experiments designed to determine the mechanisms which control the population sizes of these species in such CF cultures. One strain each of Escherichia coli, Fusobacterium sp.
View Article and Find Full Text PDFAn anaerobic continuous-flow (CF) culture method has been developed which reproduces a number of bacterial interactions that occur in the large intestine of mice. These were determined in the following ways. (i) Bacterial counts in smears stained with 37 specific fluorescent antisera showed that the numeric balance between 37 strict anaerobes isolated from conventional mice was maintained in CF culture of conventional mouse flora in the same manner as in conventional mice.
View Article and Find Full Text PDFAppl Environ Microbiol
August 1977
The microbial flora of the nose, throat, and feces of male beagle dogs housed in a "locked environment" (i.e. confined to germfree-style isolators and supplied with sterile food, air, and water) or an open environment were assessed between 26 and 30 months into the study.
View Article and Find Full Text PDFAppl Environ Microbiol
August 1977
Nine male beagle dogs, housed in either a conventional or locked environment for 2.5 years, were killed, and the bacterial flora present in various regions of each gastrointestinal tract was assessed by culture techniques, light microscopy, and scanning electron microscopy. All dogs possessed a complex microflora in their colons; in almost every dog anaerobes predominated.
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