Covalent attachment of insulin to the outer surface of liposomes.

We describe a method for covalent binding of insulin to the outer surface of multilamellar liposomes loaded with spin label. Encapsulation of the label Tempocholine-nitroxide within the aqueous phases of liposomes is controlled by Electron Spin Resonance. The binding of insulin is performed using the Carlsson's heterobifunctional reagent: N-succinimidyl 3-(2-pyridyldithio) propionate.

Histogenesis of the endocrine pancreas in newborn rats after destruction by streptozotocin. An immunocytochemical study.

Endocrine pancreatic tissue in newborn rats was studied 1 to 17 days after the destruction of B cells by an injection of streptozotocin. Regeneration of insulin cells was observed four days after streptozotocin injection, which was followed by recovery from the diabetic state and an increased pancreatic insulin content. Regeneration was characterised by new islets budding from small ducts.