The proximity interactome of PML isoforms I and II under fatty acid stress.

Promyelocytic leukemia (PML) protein forms the scaffold for PML nuclear bodies (PML NB) that reorganize into Lipid-Associated PML Structures (LAPS) under fatty acid stress. We determined how the fatty acid oleate alters the interactome of PMLI or PMLII by expressing fusions with the ascorbate peroxidase APEX2 in U2OS cells. The resultant interactome included ESCRT and COPII transport protein nodes.

Impact of a tailored exercise regimen on physical capacity and plasma proteome profile in post-COVID-19 condition.

Background: Individuals affected by the post-covid condition (PCC) show an increased fatigue and the so-called post-exertion malaise (PEM) that led health professionals to advise against exercise although accumulating evidence indicates the contrary. The goal of this study is to determine the impact of a closely monitored 8-week mixed exercise program on physical capacity, symptoms, fatigue, systemic oxidative stress and plasma proteomic profiles of PCC cases.

Methods: Twenty-five women and men with PCC were assigned sequentially to exercise ( = 15) and non-exercise ( = 10) groups.

The upper limit of thermoneutrality is not indicative of thermotolerance in bats.

To assess the vulnerability of birds and mammals to climate change recent studies have used the upper critical limit of thermoneutrality (T) as an indicator of thermal tolerance. But, the association between T and thermal tolerance is not straightforward and most studies describe T based solely on a deviation in metabolism from basal levels, without also considering the onset of evaporative cooling. It was argued recently that certain torpor-using bat species who survived prolonged exposure to high ambient temperatures (i.

Assessing responses to heat in a range-shifting, nocturnal, flying squirrel.

Over the last few decades North American flying squirrels ( spp.) have experienced dramatic northward range shifts. Previous studies have focused on the potential effects of warming winter temperatures, yet the hypothesis that rising summer temperature had a role in these range shifts remained unexplored.

Reporting guidelines for terrestrial respirometry: Building openness, transparency of metabolic rate and evaporative water loss data.

Respirometry is an important tool for understanding whole-animal energy and water balance in relation to the environment. Consequently, the growing number of studies using respirometry over the last decade warrants reliable reporting and data sharing for effective dissemination and research synthesis. We provide a checklist guideline on five key sections to facilitate the transparency, reproducibility, and replicability of respirometry studies: 1) materials, set up, plumbing, 2) subject conditions/maintenance, 3) measurement conditions, 4) data processing, and 5) data reporting and statistics, each with explanations and example studies.