Publications by authors named "D C Bubnov"
CRISPR/Cas9-based technologies are revolutionising the way we engineer microbial cells. One of the key advantages of CRISPR in strain design is that it enables chromosomal integration of marker-free DNA, eliminating laborious and often inefficient marker recovery procedures. Despite the benefits, assembling CRISPR/Cas9 editing systems is still not a straightforward process, which may prevent its use and applications.
View Article and Find Full Text PDFCRISPR/Cas9-based technologies are revolutionising the way we engineer microbial cells. One of the key advantages of CRISPR in strain design is that it enables chromosomal integration of marker-free DNA, eliminating laborious and often inefficient marker recovery procedures. Despite the benefits, assembling CRISPR/Cas9 editing systems is still not a straightforward process, which may prevent its use and applications.
View Article and Find Full Text PDFIn the current study, we report the identification and characterization of the gene product as a novel amino acid carrier in K-12 cells. Both phenotypic and biochemical analyses showed that YifK acts as a permease specific to L-threonine and, to a lesser extent, L-serine. An assay of the effect of uncouplers and composition of the reaction medium on the transport activity indicates that YifK utilizes a proton motive force to energize substrate uptake.
View Article and Find Full Text PDFAim: To evaluate the effectiveness of a new system for telemetric electrocardiogram (ECG) monitoring in patients after endovascular interventions (EI) on the coronary arteries (CA).
Materials And Methods: 168 patients with chronic ischemic heart disease who underwent EI on the CA on an outpatient basis, and during routine hospitalization, followed by telemetric ECG-monitoring after interventions were included. The monitoring was carried out using a three-channel telemetric recorder Astrocard HE3 (Russia), which provides continuous monitoring of 3-lead ECG for a long time.
Article Synopsis
- Advances in bacterial genome engineering face challenges with delivering large synthetic constructs, prompting the need for improved methods.
- This study introduces a robust method for markerless integration of DNA fragments that encode entire metabolic pathways using λRed recombineering and a CI repressor-based counterselection strategy.
- The technique enhances integration efficiency (50-100%) by utilizing the Ocr antirestriction function of T7 phage, making it applicable to multiple enterobacterial species and facilitating straightforward assembly of mutations in strains like Escherichia coli.