The effects of a stimulating intron on the expression of heterologous genes in Arabidopsis thaliana.

Introns are often added to transgenes to increase expression, although the mechanism through which introns stimulate gene expression in plants and other eukaryotes remains mysterious. While introns vary in their effect on expression, it is unknown whether different genes respond similarly to the same stimulatory intron. Furthermore, the degree to which gene regulation is preserved when expression is increased by an intron has not been thoroughly investigated.

Antimicrobial resistance of bacterial agents of the upper respiratory tract in South Indian population.

The study was aimed at determining bacterial agents of the upper respiratory tract and the susceptibility patterns of isolates to antibiotics. The throat swab samples from 250 patients suspected of upper respiratory tract infection (URTI) were obtained from the General Medicine outpatient department of a Rural Health Centre of Rajah Muthiah Medical College and Hospital (RMMC and H), Annamalai University, Chidambaram, Tamilnadu, India and inoculated in the culture medium. The bacterial infection was confirmed only in 228 patients.

Small molecules with antimicrobial activity against E. coli and P. aeruginosa identified by high-throughput screening.

Background And Purpose: New antimicrobials are needed because of the emergence of organisms that are resistant to available antimicrobials. The purpose of this study was to evaluate a high-throughput screening approach to identify antibacterials against two common disease-causing bacteria, and to determine the frequency, novelty, and potency of compounds with antibacterial activity.

Experimental Approach: A high-throughput, turbidometric assay of bacterial growth in a 96-well plate format was used to screen a diverse collection of 150,000 small molecules for antibacterial activity against E.

Chronic lymphocytic leukaemia presenting as chronic generalised erythroderma.

A 40-year-old lady presented with pyrexia of unknown origin and chronic generalised erythroderma. The blood picture was of leukaemia and the cutaneous histopathology showed leukaemic infiltration of the dermis.