The optogenetic tool LEXY consists of the second light oxygen voltage (LOV) domain of phototropin 1 mutated to contain a nuclear export signal. It allows exporting from the nucleus with blue light proteins of interest (POIs) genetically fused to it. Mutations slowing the dark recovery rate of the LOV domain within LEXY were recently shown to allow for better depletion of some POIs from the nucleus in embryos and for the usage of low light illumination regimes.
View Article and Find Full Text PDFSynthetic riboswitches gain increasing interest for controlling transgene expression in diverse applications ranging from synthetic biology, functional genomics, and pharmaceutical target validation to potential therapeutic approaches. However, existing systems often lack the pharmaceutically suited ligands and dynamic responses needed for advanced applications. Here we present a series of synthetic riboswitches for controlling gene expression through the regulation of alternative splicing.
View Article and Find Full Text PDFDefective DNA damage response (DDR) pathways are enabling characteristics of cancers that not only can be exploited to specifically target cancer cells but also can predict chemotherapy response. Defective Homologous Recombination Repair (HRR) function, e.g.
View Article and Find Full Text PDFRationale: An argon inverse low-temperature plasma (iLTP) ionization source for liquid chromatography/tandem mass spectrometry was developed. The iLTP is constructed from simple chromatographic supply materials and is implemented into an atmospheric pressure chemical ionization (APCI) source replacing the APCI discharge needle electrode. The newly developed ion source was coupled to an ultra-high-performance liquid chromatography (UHPLC) system.
View Article and Find Full Text PDFRapid Commun Mass Spectrom
September 2020
Rationale: High-throughput liquid chromatography/mass spectrometry (LC/MS) is an increasing topic in analytical chemistry. Especially the idle time of a mass spectrometer should be reduced for an efficient and cost-saving use. Therefore, a fast-switching dual ion source was developed, which uses the most important ionization techniques at atmospheric pressure, electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI), with one or more LC systems.
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