Comparison of two blastocyst culture systems: coculture on Vero cells and sequential media.

Objective: To compare two blastocyst culture systems: culture on Vero cells and sequential media in the same time period.

Design: Retrospective study.

Setting: Institutional practice assisted reproductive technology center.

[Extra-uterine pregnancies after in vitro fertilization. Six case reports].

Ectopic pregnancies after in vitro fertilization (IVF) are more frequent because of the development of assisted reproductive technology. This retrospective study analysed 6 cases of ectopic pregnancy after IVF from the "clinique du Parc" (Tours) during a 3-year period. Tubal disease was found to be the main risk-factor for ectopic pregnancy after IVF heterotopic pregnancy may occur and its diagnosis is often difficult.

Uterine rupture in first or second trimester of pregnancy after in-vitro fertilization and embryo transfer.

We report five cases of early rupture of cornual pregnancy with history of previous salpingectomy and cornual resection following in-vitro fertilization (IVF) and embryo transfer. We discuss the predisposing factors, diagnostic and therapeutic modalities in these patients. A high index of suspicion is required for an early diagnosis.

Morphological and cytogenetic observations of unfertilized human oocytes and abnormal embryos obtained after ovarian stimulation with pure follicle stimulating hormone following pituitary desensitization.

Morphological and cytological observations of 189 unfertilized oocytes and 40 abnormal embryos obtained from 32 patients in a routine in-vitro fertilization programme were performed. Both the oocytes and the embryos were mounted whole to preserve the original topology of all the structural elements. With the applied protocol of ovarian stimulation associating pituitary desensitization and follicle stimulating hormone stimulation, a high degree of immaturity of the unfertilized eggs was observed in comparison with previous reports.