In-vivo Raman microspectroscopy reveals differential nitrate concentration in different developmental zones in Arabidopsis roots.

Background: Nitrate (NO) is one of the two major forms of inorganic nitrogen absorbed by plant roots, and the tissue nitrate concentration in roots is considered important for optimizing developmental programs. Technologies to quantify the expression levels of nitrate transporters and assimilating enzymes at the cellular level have improved drastically in the past decade. However, a technological gap remains for detecting nitrate at a high spatial resolution.

Real-Time Quantification of Molecular-Level Dynamic Behaviors Underpinning Shear Thinning in End-Linked Associative Polymer Networks.

Shear thinning of associative polymers is tied to bond breakage under deformation and retraction of dangling chains, as predicted by transient network theories. However, an in-depth understanding of the molecular mechanisms is limited by our ability to measure the molecular states of the polymers during deformation. Herein, utilizing a custom-built rheo-fluorescence setup, bond dissociation in model end-linked associative polymers is quantified in real time with nonlinear shear deformation based on a fluorescence quench transition when phenanthroline ligands bind with Ni.

The relationship between periodontal status, physical activity, dietary practices, and dental caries.

The objective of this study is to evaluate the correlation between dietary patterns, levels of physical activity (PA), and the occurrence of periodontal disease. Many lifestyles have been connected to periodontal disease, but little is known about diet and PA intake. This study included 185 people-62 healthy, 61 gingivitis, and 62 periodontitis.

Impact of platelet-rich fibrin derivatives on patient morbidity and quality of life in palatal donor sites following free gingival graft surgery: a randomized clinical trial.

Objectives: Platelet concentrates are biomaterials with significant potential in tissue regeneration, functioning as scaffolds with greater leukocyte inclusion and a flexible fibrin mesh. However these concentrates have different preparation methods and biological properties. The objective of this clinical investigation was to evaluate the effects of utilizing platelet-rich fibrin (PRF) materials (L-PRF and A-PRF) as a palatal bandage following free gingival graft (FGG) on patients' morbidity and oral health-related quality of life.

Quantum-Enhanced Detection of Viral cDNA via Luminescence Resonance Energy Transfer Using Upconversion and Gold Nanoparticles.

The COVID-19 pandemic has profoundly impacted global economies and healthcare systems, revealing critical vulnerabilities in both. In response, our study introduces a groundbreaking method for the detection of SARS-CoV-2 cDNA, leveraging Luminescence resonance energy transfer (LRET) between upconversion nanoparticles (UCNPs) and gold nanoparticles (AuNPs) to achieve an unprecedented detection limit of 242 femtomolar (fM). This innovative sensing platform utilizes UCNPs conjugated with one primer and AuNPs with another, targeting the 5' and 3' ends of the SARS-CoV-2 cDNA, respectively, enabling precise differentiation of mismatched DNA sequences and significantly enhancing detection specificity.