A De Novo Designed Trimeric Metalloprotein as a Ni Model of the Acetyl-CoA Synthase.

We present a Ni site model of acetyl coenzyme-A synthase (ACS) within a de novo-designed trimer peptide that self-assembles to produce a homoleptic Ni(Cys) binding motif. Spectroscopic and kinetic studies of ligand binding demonstrate that Ni binding stabilizes the peptide assembly and produces a terminal Ni-CO complex. When the CO-bound state is reacted with a methyl donor, a new species is quickly produced with new spectral features.

Problems faced by Malaysians during the Movement Control Order and Conditional Movement Control Order: A cross-sectional study.

Introduction: The COVID-19 pandemic had caused Malaysia to introduce a lockdown approach for the first time that was due to an outbreak of infectious disease. This was bound to create certain problems as it disrupts Malaysians' daily routine and way of life.

Materials And Methods: A cross-sectional study to identify the problems faced by Malaysian Social Media Users during the Movement Control Order (MCO) and Conditional Movement Control Order (CMCO) was conducted.

A De Novo-Designed Artificial Metallopeptide Hydrogenase: Insights into Photochemical Processes and the Role of Protonated Cys.

Hydrogenase enzymes produce H gas, which can be a potential source of alternative energy. Inspired by the [NiFe] hydrogenases, we report the construction of a de novo-designed artificial hydrogenase (ArH). The ArH is a dimeric coiled coil where two cysteine (Cys) residues are introduced at tandem a/d positions of a heptad to create a tetrathiolato Ni binding site.

The Medium-Term Survival Analysis of an All-Polyethylene Tibia in a Single-Series Cohort of Over 1000 Knees.

Background: Total knee arthroplasty is a successful elective orthopedic procedure with an increasing number being undertaken. Original knee arthroplasties used an all-polyethylene tibia; however, with concerns over tibial loosening, the trend moved toward the metal-backed variety. Modern designs providing more conformity and changes in manufacturing of the polyethylene make it an equivalent but cheaper option.

Biosynthetic Approaches towards the Design of Artificial Hydrogen-Evolution Catalysts.

Hydrogen is a clean and sustainable form of fuel that can minimize our heavy dependence on fossil fuels as the primary energy source. The need of finding greener ways to generate H gas has ignited interest in the research community to synthesize catalysts that can produce H by the reduction of H . The natural H producing enzymes hydrogenases have served as an inspiration to produce catalytic metal centers akin to these native enzymes.