Publications by authors named "D Allman"
Innate-like splenic marginal zone (MZ) B (MZB) cells play unique roles in immunity due to their rapid responsiveness to blood-borne microbes. How MZB cells integrate cell-extrinsic and -intrinsic processes to achieve accelerated responsiveness is unclear. We found that Delta-like1 (Dll1) Notch ligands in splenic fibroblasts regulated MZB cell pool size, migration, and function.
View Article and Find Full Text PDFArticle Synopsis
- During B-cell development, the pro-B-cell stage is crucial for committing to the B-cell lineage, and the transcription factor YY1 plays a key role in this process.
- Knocking out YY1 at the pro-B-cell stage stops B-cell development and enables these cells to convert into T lineage cells, demonstrating a shift in their genetic expression profiles.
- Research involving various sequencing techniques shows that the loss of YY1 not only affects B lineage commitment but also allows pro-B cells to exhibit flexibility in developing into multiple hematopoietic lineages due to changes in chromatin accessibility.
Multiple myeloma is a largely incurable and life-threatening malignancy of antibody-secreting plasma cells. An effective and widely available animal model that recapitulates human myeloma and related plasma cell disorders is lacking. We show that busulfan-conditioned human IL-6-transgenic (hIL-6-transgenic) NSG (NSG+hIL6) mice reliably support the engraftment of malignant and premalignant human plasma cells, including from patients diagnosed with monoclonal gammopathy of undetermined significance, pre- and postrelapse myeloma, plasma cell leukemia, and amyloid light chain amyloidosis.
View Article and Find Full Text PDFArticle Synopsis
- - The pro-B cell stage is crucial for commitment to the B cell lineage, and the transcription factor YY1 plays a key role in this process; knocking out YY1 during this stage prevents cells from committing to B cells.
- - Instead of developing into B cells, YY1 knockout pro-B cells can differentiate into T lineage cells in specific experimental conditions, demonstrating their ability to switch lineages.
- - Single-cell RNA sequencing reveals that these YY1 knockout pro-B cells display a variety of transcript profiles, indicating a significant level of lineage plasticity and suggesting that YY1 is important for lineage commitment across different cell types.
Plasma cells produce large quantities of antibodies and so play essential roles in immune protection. Plasma cells, including a long-lived subset, reside in the bone marrow where they depend on poorly defined microenvironment-linked survival signals. We show that bone marrow plasma cells use the ligand-gated purinergic ion channel P2RX4 to sense extracellular ATP released by bone marrow osteoblasts through the gap-junction protein pannexin 3 (PANX3).
View Article and Find Full Text PDF