Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants.

Pseudomonas aeruginosa is a phenotypically and genotypically diverse and adaptable Gram-negative bacterium ubiquitous in human environments. P. aeruginosa is able to form biofilms, develop antibiotic resistance, produce virulence factors, and rapidly evolve in the course of a chronic infection.

The self-curing phenomenon of schistosome infection in rhesus macaques: insight from in vitro studies.

A reduction in the burden of schistosomiasis is potentially achievable by integrating a schistosomiasis vaccine with current control measures. Here, we determine parasite-specific in vitro responses of B, T, and NK cells from naive uninfected rhesus macaques to Schistosoma mansoni (Sm) egg (SmEA) and worm antigen (SmWA) preparations isolated from infected baboons. Pronounced B cell responses to SmEA and NK cell responses to both SmEA and SmWA were observed.

In vivo reflectance confocal microscopy of basal cell carcinoma with cystic degeneration.

Basal cell carcinoma (BCC) is the most common skin cancer, may display various clinical aspects, frequently has subclinical extension, and through local invasion may induce important tissue damage. Reflectance confocal microscopy (RCM) is a modern technique that allows a non-invasive investigation of skin structure with a nearly histological resolution, and may achieve an optimal in vivo evaluation of skin tumors in combination with dermoscopy. We report a case of nodular BCC with areas of cystic degeneration in which in vivo RCM and dermoscopy evaluation enabled an accurate diagnosis and a detailed preoperative evaluation of tumor margins allowing the optimization of surgical treatment, with best aesthetic and functional results.

The role of confocal microscopy in the dermato-oncology practice.

Reflectance-mode confocal microscopy (RCM) is a new in vivo skin imaging technique. We present our one-year experience in RCM examinations in skin tumors and the retrospective analysis of patients enrolled in the Dermatological Department of 'N. Paulescu' Institute using the Fotofinder Dermoscope IIŴ for the dermatoscopy analysis and VivaScope 1500Ŵ for in vivo RCM.