Publications by authors named "D Aishwarya"
Article Synopsis
- The study investigates the degradation products of antidiabetic drugs glimepiride (GMD) and glyburide (GBD) and their potential genotoxicity after exposure to stressors like acid, base, and hydrogen peroxide.
- Through advanced techniques like semi-preparative HPLC and LC-Orbitrap MS, researchers identified and isolated various impurities of both drugs, finding a total of five GMD and three GBD impurities, with specific purity levels determined.
- The in silico predictions indicated low genotoxic potential for most impurities, but in vitro comet assays showed that some impurities, particularly from GBD, exhibited genotoxicity, with one impurity showing a higher level than the others.
Glioblastoma, which affects a large number of patients every year and has an average overall lifespan of around 14.6 months following diagnosis stands out as the most lethal primary invasive brain tumor. Currently, surgery, radiation, and chemotherapy with temozolomide (TMZ) are the three major clinical treatment approaches.
View Article and Find Full Text PDFAffinity-based protein depletion and TiO enrichment methods play a crucial role in detection of low-abundant proteins and phosphopeptides enrichment, respectively. Here, we assessed the effectiveness of HSA/IgG (HU2) and Human 7 (HU7) depletion methods and their impact on phosphopeptides coverage through comparative proteome analysis, utilizing in-solution digestion and nano-LC-Orbitrap mass spectrometry (MS). Our results demonstrated that both HU2 and HU7 affinity depletion significantly decreased high-abundant proteins by 1.
View Article and Find Full Text PDFArticle Synopsis
- N-Nitroso dimethylamine (NDMA) is a harmful impurity found in ranitidine products, with the potential to form during storage due to the drug's chemical structure, prompting the FDA to recommend antioxidants as a solution.
- A sensitive liquid chromatography/high-resolution mass spectrometry (LC-HRMS) method was developed for detecting NDMA, achieving high sensitivity and precise quantification of NDMA levels in ranitidine samples.
- The study confirmed that antioxidants effectively reduce NDMA formation during nitrosation of ranitidine, with ascorbic acid being the most effective, significantly lowering NDMA levels by nearly 97%.
Article Synopsis
- The study found that caprylic acid (C8), a medium-chain fatty acid, is highly effective against Leishmania donovani, the cause of visceral leishmaniasis.
- Researchers identified ergosterol depletion as the main cause of cell death induced by caprylic acid and its analogs, targeting the enzyme mevalonate kinase (MevK) in the ergosterol synthesis pathway.
- This research provides new insights into a natural compound's mechanism against a pathogen, highlighting the potential for alternative treatments in the context of drug resistance.