Publications by authors named "D Ahern"
Article Synopsis
- - Kisspeptin plays a crucial role in regulating gonadotropin secretion in pigs, and a study was conducted on F1 pigs from a parental line created by CRISPR/Cas9 knockout of the KISS1 gene, which eliminates the need for castration.
- - The research measured body weights, gonadotropin levels, and reproductive development in these pigs, finding that while body weight didn't differ based on genotype, KISS1-/- boars and gilts exhibited significantly reduced gonadotropin concentrations and experienced hypogonadism.
- - The results suggest that only one functional KISS1 allele is necessary for normal gonadal function and hormone secretion, and that the knockout of KISS1 does
Article Synopsis
- - Excess gene dosage from chromosome 21 is linked to Down syndrome, affecting both development and acute health issues, but it’s unclear which issues can still be addressed after development is complete.
- - Researchers created trisomy 21 (T21) human stem cells to study how silencing one chromosome 21 copy affects cell development, finding that this silencing is effective and irreversible in stem cells.
- - Inducing chr21 dosage correction before neural progenitor development helps prevent an imbalance in cell type differentiation, and importantly, the correction can be activated even in fully developed neurons and astrocytes, allowing for further investigation of specific Down syndrome traits that could still be treated.
Single cell spatial interrogation of the immune-structural interactions in COVID -19 lungs is challenging, mainly because of the marked cellular infiltrate and architecturally distorted microstructure. To address this, we develop a suite of mathematical tools to search for statistically significant co-locations amongst immune and structural cells identified using 37-plex imaging mass cytometry. This unbiased method reveals a cellular map interleaved with an inflammatory network of immature neutrophils, cytotoxic CD8 T cells, megakaryocytes and monocytes co-located with regenerating alveolar progenitors and endothelium.
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- Mass cytometry fuses the detailed analysis of mass spectrometry with the efficiency of flow cytometry, allowing researchers to examine drug effects at a single-cell level across different cell types.
- The chapter provides an overview of how to conduct mass cytometry experiments, detailing the process from staining cells to cleaning data, along with the platform's technical limitations.
- Three experiments are described: validating a metal-conjugated antibody, analyzing the effects of a kinase inhibitor on cell cycle and apoptosis in a myeloma cell line, and investigating changes in transcription factor expression in response to drug treatment in blood cells.
Introduction: Despite successful fusion rates with iliac crest bone graft (ICBG), donor-site morbidity and increased operating time remain a considerable limitation and drive the search for alternatives. In this systematic review, grafts with additional cellular supplementation were compared with ICBG for spinal arthrodesis. We compared safety, efficacy and long-term outcomes, thus providing the current and relevant evidence for orthopaedic surgeons to make informed choices regarding this rapidly developing field.
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