[Efficiency of chromatographic separation of the pigment-protein lipid complexes of the reaction centers of photosystems 1 and 2 from pea chloroplasts on DEAE-cellulose].

Using a previously developed procedure, further optimization of isolation of the functionally active pigment-protein lipid complex of the reaction center of photosystem 2 (PPLC RC PS-2) by DEAE-cellulose chromatography has been carried out. Preliminary chromatographic separation of PPLC RC PS-1 and PS-2 in the presence of a light-harvesting complex has proved to be the most efficient technique. In the latter case the admixture of P700 within PPLC RC PS-2 decreases (1.

[Optimization of isolation conditions for three types of pigment protein-lipid complexes from chloroplasts during solubilization with Triton X-100].

The maximal total release of pigment protein-lipid complexes (PPLC) during their isolation from pea chloroplasts was achieved by 1-hr solubilization with Triton X-100, the Triton:chlorophyll (T/Chl) ratio being 50 mg/mg/ml. The total yield of the reaction center complexes (sigma PPLC RC) was 22,3%, whereas that of the auxiliary light-accumulating complex (ALA-PPLC) was approximately 32% with respect to Chl. An increase in the solubilization time and of the T/Chl ratio resulted in dissociation of ALA-PPLC.