Publications by authors named "D A Winter"
Background: Eosinophilic esophagitis (EoE) is a chronic T helper type 2 (Th2)-associated inflammatory disorder triggered by food allergens, resulting in esophageal dysfunction through edema, fibrosis, and tissue remodeling. The role of epithelial remodeling in EoE pathogenesis is critical but not fully understood.
Objective: To investigate the role of epithelial IKKβ/NFκB signaling in EoE pathogenesis using a mouse model with conditional Ikkβ knockout in esophageal epithelial cells (Ikkβ).
For the reproducible analysis of peptides by mass spectrometry-based proteomics, data-independent acquisition (DIA) and parallel/multiple reaction monitoring (PRM/MRM) deliver unrivalled performance with respect to sensitivity and reproducibility. Both approaches, however, come with distinct advantages and shortcomings. While DIA enables unbiased whole proteome analysis, it shows limitations with respect to dynamic range and the quantification of low-abundant proteins.
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- Glycosylation is crucial for modifying lipids and sorting proteins, with its regulation involving a unique distribution of enzymes in the Golgi and the action of SPPL3.
- In cells lacking the retention factor LYSET/TMEM251, there is increased secretion of a Golgi protein, B4GALT5, due to disrupted M6P tagging, which typically marks proteins for lysosomal degradation.
- The study reveals that GOLPH3 and GOLPH3L adaptors play a critical role in stabilizing the LYSET-GNPT complex, maintaining proper Golgi function, and ensuring efficient lysosomal enzyme processing.
Article Synopsis
- Childhood cancer survivors (CCS) have a higher likelihood of developing dyslipidemia, which is a significant risk factor for heart disease, compared to adults without a cancer history.
- A study analyzed data from 2,338 CCS and found various lipid abnormalities in the survivors, indicating a prevalence of issues like high triglycerides and low HDL cholesterol.
- Factors influencing dyslipidemia in CCS included age, sex, BMI, physical activity, and treatment history, highlighting the need for tailored health monitoring for these individuals.