How Do Gepotidacin and Zoliflodacin Stabilize DNA-Cleavage Complexes with Bacterial Type IIA Topoisomerases? 2. A Single Moving Metal Mechanism.

DNA gyrase is a bacterial type IIA topoisomerase that can create temporary double-stranded DNA breaks to regulate DNA topology and an archetypical target of antibiotics. The widely used quinolone class of drugs use a water-metal ion bridge in interacting with the GyrA subunit of DNA gyrase. Zoliflodacin sits in the same pocket as quinolones but interacts with the GyrB subunit and also stabilizes lethal double-stranded DNA breaks.

Benchmarking DNA isolation methods for marine metagenomics.

Metagenomics is a powerful tool to study marine microbial communities. However, obtaining high-quality environmental DNA suitable for downstream sequencing applications is a challenging task. The quality and quantity of isolated DNA heavily depend on the choice of purification procedure and the type of sample.

Complete genome sequences of three commensal and two avian pathogenic strains isolated from farm animals in Russia.

Farm animals are a natural reservoir of commensal and pathogenic strains with high zoonotic potential. Here, we present five complete genomes of strains isolated from healthy animals and animals with colisepticemia from farms in Russia. The strains contain diverse virulence-associated and antibiotic resistance genes and multiple plasmids.

Bacteriocin-Producing Q5 and C41 with Potential Probiotic Properties: In Silico, In Vitro, and In Vivo Studies.

Commensal bacteriocin-producing are of interest for possible use as probiotics to selectively control the spread of pathogenic bacteria. Here, we evaluated the biosafety and efficacy of two new bacteriocin-producing strains, Q5 (VKM B-3706D) and C41 (VKM B-3707D), isolated from healthy farm animals. The genomes of both strains were sequenced, and genes responsible for the antagonistic and colonization abilities of each strain were identified.