Publications by authors named "D A Spratt"
Experimental approaches to investigate biophysical interactions between homeodomain transcription factors and DNA.
Biochim Biophys Acta Gene Regul Mech
December 2024
Article Synopsis
- Homeodomain transcription factors (TFs) bind to specific DNA sequences, influencing target gene expression, but their binding affinity and specificity remain largely unclear.
- Various qualitative methods like DNA footprinting and EMSAs are commonly used to study TF-DNA interactions, while bioinformatics also plays a role in discovering TF binding sites.
- The text highlights the pros and cons of these methods and suggests that more quantitative approaches, such as MITOMI and MST, could enhance our understanding of the biophysical aspects of TF-DNA binding and improve predictive models.
Homologous to the C-terminus of E6AP (HECT) and RCC1-like domain (RLD)-containing protein 2 (HERC2) is a large, 528 kDa E3 ubiquitin ligase that is associated with cancer, oculocutaneous albanism type 2, Prader-Willi syndrome, and other neurological diseases. HERC2 has been found to contribute to double-stranded DNA break repairs, tumor suppression, maintaining centrosome architecture, and ubiquitylation. The C-terminal portion of the HECT domain (C-lobe) of HERC2 is responsible for transferring ubiquitin to a substrate but the precise function of the other eight domains in HERC2 are unknown.
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