Publications by authors named "D A Shagin"

is an opportunistic pathogen that can cause infections in humans, especially in hospital patients with compromised host defence mechanisms, including patients with cystic fibrosis. Filamentous bacteriophages represent a group of single-stranded DNA viruses infecting different bacteria, including and other human and animal pathogens; many of them can replicate when integrated into the bacterial chromosome. Filamentous bacteriophages can contribute to the virulence of and influence the course of the disease.

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Nonfermenting gram-negative (NFGN) bacteria were isolated from cystic fibrosis (CF) patients and subjected to susceptibility testing and whole-genome sequencing. Among 170 enrolled CF patients, 112 (65.9%) were colonized with at least 1 key NFGN species.

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The clinical isolate of Klebsiella pneumoniae 1333/P225 was revealed as containing a KL108 K. pneumoniae K locus for capsule biosynthesis. The gene cluster demonstrated a high level of sequence and arrangement similarity with that of the E.

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Article Synopsis
  • Understanding the diversity of capsular polysaccharides (CPS) is essential for effectively using phage therapy against panresistant Acinetobacter baumannii infections.
  • The clinical isolate A. baumannii 36-1454, which has a novel K locus (KL127), shows differences in CPS structure compared to another strain (KL128), specifically in the glycosyltransferase genes and linkages of sugar units in their CPS chains.
  • The study highlights the relevance of Wzy proteins in forming CPS linkages and discusses how these findings can inform future phage therapy strategies for treating infections that are resistant to current antibiotics.
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The pneumococcal population structure and drug resistance patterns are constantly changing worldwide. In this study, we described serotypes and antimicrobial susceptibility among 478 multiple-drug resistant (MDR) pediatric nasopharyngeal pneumococci recovered in 2010-2017. The majority of isolates (89.

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