Transcriptome analysis reveals multiple targets of erythritol-related transcription factor EUF1 in unconventional yeast Yarrowia Lipolytica.

Background: Erythritol is a four-carbon polyol with an unclear role in metabolism of some unconventional yeasts. Its production has been linked to the osmotic stress response, but the mechanism of stress protection remains unclear. Additionally, erythritol can be used as a carbon source.

Mutation in yl-HOG1 represses the filament-to-yeast transition in the dimorphic yeast Yarrowia lipolytica.

Background: Yarrowia lipolytica is a dimorphic fungus, which switches from yeast to filament form in response to environmental conditions. For industrial purposes it is important to lock cells in the yeast or filamentous form depending on the fermentation process. yl-Hog1 kinase is a key component of the HOG signaling pathway, responsible for activating the osmotic stress response.

In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica.

The unconventional yeast Yarrowia lipolytica produces erythritol as an osmoprotectant to adapt to osmotic stress. In this study, the array of putative erythrose reductases, responsible for the conversion of d-erythrose to erythritol, was analyzed. Single knockout and multiple knockout strains were tested for their ability to produce polyols in osmotic stress conditions.

The Overexpression of Results in Enhanced Erythritol Synthesis from Glycerol by the Yeast .

The unconventional yeast is used to produce erythritol from glycerol. In this study, the role of the erythrose reductase (ER) homolog in erythritol synthesis was analyzed. The deletion of the gene resulted in an increased production of mannitol (308%) and arabitol (204%) before the utilization of these polyols began.

HOG-Independent Osmoprotection by Erythritol in Yeast .

Erythritol is a polyol produced by under hyperosmotic stress. In this study, the osmo-sensitive strain was subjected to stress, triggered by a high concentration of carbon sources. The strain thrived on 0.