Tumor necrosis factor beta gene polymorphism and early primary kidney allograft loss.

The purpose of this study was to examine the association of TNFB alleles and genotypes in recipients experiencing early cadaveric kidney graft loss and non-early graft loss. Because the TNFB1 allele is associated with increased production of the TNF beta lymphotoxin following PHA mitogenic stimulation in vitro, the frequency of this allele was examined in renal transplant recipients experiencing early graft loss. A similar expression of TNFB alleles and genotypes was found in the early regraft loss group (< or = 3 months; n = 16) and non-early graft loss group (n = 81).

Identification of high- and low-risk second kidney grafts.

The purpose of this study was to identify recipients who are at low or high risk of early cadaveric regraft failure by segregating results of the flow cytometric crossmatch (FCXM) test with previous graft survival time (PGST). Early immunologic kidney regraft failure was analyzed in 103 multicenter recipients by cross-stratifying FCXM negative/positive status with < or =3- and >3-month PGST. T cell and B cell cytotoxicity crossmatches were negative.

Intradialytic granulocyte reactive oxygen species production: a prospective, crossover trial.

By the use of flow cytometric techniques, this prospective, randomized crossover study was designed to analyze intradialytic granulocyte reactive oxygen species (ROS) formation in whole blood with complement-activating and noncomplement-activating hollow fiber membranes. Dialysis with a complement-activating membrane resulted in a 6.5-fold increase in granulocyte hydrogen peroxide production 15 min after dialysis initiation and remained significantly elevated (P < 0.

Detection of granulocyte reactive oxygen species formation in whole blood using flow cytometry.

We have developed a technique for analysis of granulocyte reactive oxygen species formation in whole blood using flow cytometry and two color immunofluorescence. This technique relies upon the use of specific fluorescent dye (LDS-751) to stain nucleated cells, eliminating erythrocytes from analysis. Using LDS-751, forward angle light scatter, and 90 degrees side scatter, a granulocyte gate, monocyte gate, and lymphocyte gate were identified.