Sugar Detection in Mosquitoes via Anthrone Tests.

Adult mosquitoes feed on plant sugar, frequently in the form of floral nectar. However, because of spatial and temporal variation in this behavior, as well as to the predilection of most mosquitoes to alter their behavior in the presence of a researcher, observing mosquito nectar feeding and similar behaviors directly in real time is not always feasible. In this protocol, I describe methods for hot and cold anthrone tests, which can be used to quantify the degree to which mosquito sugar feeding occurs in nature.

Measuring Mosquito Spectral Sensitivity Using Electroretinograms.

Mosquitoes use a variety of cues to locate resources in the world around them, including olfactory, thermal, and visual stimuli. Understanding how mosquitoes perceive these stimuli is important for understanding and investigating mosquito behaviors and mosquito ecology. Specifically, mosquito vision can be studied in many ways, including by using electrophysiological recordings from their compound eyes.

Mosquito (Diptera: Culicidae) Vision and Associated Electrophysiological Techniques.

Mosquitoes are considered the world's deadliest animal because of the pathogens they spread. Additionally, they are an unbearable nuisance in many areas. Visual stimuli play an important role in the mosquito life cycle, helping them find vertebrate hosts, floral nectar, and oviposition sites.

Mosquito Pollination and Sugar Detection Methods: An Overview.

The interactions between mosquitoes and plants and, particularly, between mosquitoes and plant sugars from flowers and other structures are often overlooked and are vastly underresearched compared to mosquito-vertebrate or mosquito-pathogen interactions. Given the importance of mosquito nectar-feeding behavior, as well as its impact on vectorial capacity and its implications for vector suppression, a better understanding of mosquito-plant interactions is needed. Direct observation of mosquitoes visiting plants to obtain sugar and other nutrients can be difficult because females may leave flowers to seek a blood meal from the observer, but this can be overcome with the right experimental procedures.