Publications by authors named "D A Franciscato"
A ranelate ion, an old antiosteoporotic drug, readily reacts with gold(III) ions generating stable gold nanoparticles (AuNP@Ran). The reaction proceeds rapidly under green conditions and is specific for gold ions at room temperature. Their characteristic color associated with the plasmonic resonance effects allows its use in colorimetric/spectrophotometric analysis, as well as in spot test assays.
View Article and Find Full Text PDFEvaluation of sperm head dimensions and chromatin integrity of epididymal sperm from domestic cats using the toluidine blue technique.
Anim Reprod Sci
October 2018
When using assisted reproductive technologies, there is seldom an evaluation of DNA integrity during sperm analysis, which is an important variable for proper embryo development. The toluidine blue staining technique allows the simultaneous evaluation of sperm chromatin and sperm head dimensions. The objectives of this study were to evaluate the applicability of the toluidine blue staining method for analyzing DNA abnormalities in epididymal sperm (from the caput, corpus, and cauda) of cats and to investigate the correlations among DNA condensation, morphology, and sperm head dimensions.
View Article and Find Full Text PDFThiosemicarbazone@Gold nanoparticle hybrid as selective SERS substrate for Hg ions.
Spectrochim Acta A Mol Biomol Spectrosc
November 2018
Article Synopsis
- The presence of gold nanoparticles changes the Raman spectral profile of p-methylcarbohydrazonethioamide (MCHT) due to strong Surface-Enhanced Raman Scattering (SERS) effects.
- Most metal ions do not show SERS response, indicating that bonding sites on the MCHT are occupied by gold nanoparticles, making them unavailable for other metal ion binding, except for Hg(II) ions.
- The enhancement of specific vibrational modes when Hg(II) binds to the MCHT-AuNP hybrid suggests significant conformational changes, supporting its use as a highly selective and sensitive SERS substrate.
The aim of this study was to evaluate the chromatin packing and sperm head morphometry of cryopreserved semen of Nelore bulls (Bos taurus indicus) of different ages. Furthermore, the influence of the degree of chromatin compaction on in vitro embryo production (IVP) was investigated. Forty bulls were divided into three groups: young (1.
View Article and Find Full Text PDFThe aim of this study was to evaluate apoptosis and parasite load in the liver and spleen of dogs with visceral leishmaniosis (VL), using immunohistochemistry. Liver and spleen samples from 71 dogs with VL were used. The parasite load in the spleen and liver showed significant difference between organs in infected group (P=0.
View Article and Find Full Text PDF