A ten-year retrospective on the efficacy of droplet vitrification for cryobanking of Allium ramosum L. germplasm.

Background: Allium ramosum is an important member of the genus Allium, which is commonly known as Chinese chive or fragrant-flowered garlic. Conserving the genetic diversity of different species of Allium is crucial, and cryopreservation has emerged as an important strategy for long-term conservation of alliums.

Objective: To develop a reliable protocol for the cryoconservation of A.

Cryopreservation protocol for cowpea pollen storage.

Background: Long-term storage of cowpea pollen is important for the fertilization of spatially or temporally isolated female parents, especially during cowpea crop improvement and wide hybridization programs.

Objective: Experiments were conducted to determine pollen longevity at different storage temperatures and to develop a cryopreservation protocol for pollen of different cowpea accessions.

Materials And Methods: The investigation was carried out at the Research Farm of ICAR-NBPGR, New Delhi, India, during the kharif (rainy) season of 2022.

Prediction of primary Hypertension in Primary Health Care Settings in Coastal Karnataka Using Artificial Neural Network.

Background: Hypertension, characterized by chronically elevated blood pressure, poses a significant global health burden. Its prevalence, a critical public health concern, necessitates ac-curate prediction models for timely intervention and management.

Aim: The proposed approach leverages the capability of an Artificial Neural Network to capture complex patterns and non-linear relationships within the time series data, allowing for the devel-opment of a robust forecasting model to predict Hypertension.

Revitalizing maize downy mildew management: harnessing new-generation fungicides and host plant resistance.

Background: Maize Downy Mildew (MDM) is a devastating disease in the humid sub-tropical/tropical regions of Asia. In this study, the prevalence of MDM during the rainy Kharif seasons of south Karnataka state (India) ranged between 6.8% (2018) and 19.

DNA Mimic Foldamer Recognition of a Chromosomal Protein.

Helical aromatic oligoamide foldamers bearing anionic side chains that mimic the overall shape and charge surface distribution of DNA were synthesized. Their interactions with chromosomal protein Sac7d, a non-sequence-selective DNA-binder that kinks DNA, were investigated by Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Circular Dichroism spectroscopy (CD), melting curve analysis, Atomic Force Microscopy (AFM), and Nuclear Magnetic Resonance (NMR), as well as by single crystal X-ray crystallography. The foldamers were shown to bind to Sac7d better than a DNA duplex of comparable length.