Publications by authors named "D A Dartt"
Article Synopsis
- - The study investigates the effectiveness of a 14-day treatment using topical dexamethasone and artificial tears for patients with dry eyes, measuring clinical response using various eye assessments before and after treatment.
- - A total of 167 patients (mostly women, average age 54) showed significant improvement in symptoms and objective measures like Ocular Surface Disease Index (OSDI) and Dry Eye Questionnaire (DEQ-5) one month post-treatment.
- - The findings indicate that changes in symptoms correlate with baseline measurements of dry eye disease severity, while intraocular pressure slightly decreased, showing potential effectiveness of the corticosteroid regimen in a real-world clinical context.
Article Synopsis
- The study investigates the role of conjunctival goblet cells (CjGCs) in eye health, focusing on their secretions beyond mucin, specifically extracellular vesicles (EVs) and lipid mediators during allergic inflammation.
- Findings reveal that female CjGCs significantly increase pro-resolving mediators LXA4 and RvD1 in EVs after histamine stimulation, while no such increase is observed in male CjGCs.
- This indicates a potential sex difference in allergic responses that could explain males' higher susceptibility to severe allergic conjunctivitis, suggesting new avenues for clinical treatment.*
Purinergic agonists increase [Ca] in rat conjunctival goblet cells through ryanodine receptor type 3.
Am J Physiol Cell Physiol
September 2024
ATP and benzoylbenzoyl-ATP (BzATP) increase free cytosolic Ca concentration ([Ca]) in conjunctival goblet cells (CGCs) resulting in mucin secretion. The purpose of this study was to investigate the source of the Ca mobilized by ATP and BzATP. First-passage cultured rat CGCs were incubated with Fura-2/AM, and [Ca] was measured under several conditions with ATP and BzATP stimulation.
View Article and Find Full Text PDFPurpose: To compare the effect of the ocular antiseptic treatments 0.05% chlorhexidine, 5% povidone-iodine (PI) and 5% betadine on cell viability and mucin secretion of primary cultured human goblet cells (GCs).
Method: GC viability was analysed using lactate dehydrogenase (LDH) and tetrazolium dye (MTT) colorimetric assays.
We used cultured human conjunctival goblet cells to determine (i) whether the toxigenic induced activation of the epithelial goblet cells requires two signals to activate the NLRP3 inflammasome, (ii) if one signal is mediated by TLR1, TLR2, or TLR6, and (iii) if the toxin α toxin is another signal for the activation of the inflammasome and secretion of mature IL-1β. Cultured cells were incubated with siRNA to knock down the different TLRs. After stimulation with toxigenic RN6390, pro-IL-1β synthesis, caspase-1 activity, and mature IL-1β secretion were measured.
View Article and Find Full Text PDF