Publications by authors named "D A Bolotin"
Allelic variability in the adaptive immune receptor loci, which harbor the gene segments that encode B cell and T cell receptors (BCR/TCR), is of critical importance for immune responses to pathogens and vaccines. Adaptive immune receptor repertoire sequencing (AIRR-seq) has become widespread in immunology research making it the most readily available source of information about allelic diversity in immunoglobulin (IG) and T cell receptor (TR) loci. Here, we present a novel algorithm for extrasensitive and specific variable (V) and joining (J) gene allele inference, allowing the reconstruction of individual high-quality gene segment libraries.
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- A study was conducted to analyze how often dermatologic surgeons face challenges in identifying the locations of skin cancer tumors before surgery.
- Out of over 17,000 cases, only 0.60% involved uncertain sites, with these cases more common in older, male patients who had biopsies done over a month prior.
- Surgeons commonly used various methods to confirm tumor locations, achieving success in identifying 82% of these uncertain sites, while some surgeries were delayed, especially for non-facial tumors.
Article Synopsis
- Mohs micrographic surgery (MMS) is an effective treatment for melanoma in situ (MIS), but inconsistent surgical techniques make it hard to apply current data broadly.
- To standardize MMS techniques for MIS in future studies, a modified Delphi method was used, involving expert input through voting on various surgical approaches.
- The study generated 8 consensus recommendations, with five agreed upon in the first round and the others in the second, aimed at providing uniform guidelines to improve future clinical trials.