Volatiles organic compounds in craft beers produced in Basilicata (Southern Italy).

The volatiles organic compounds of 21 samples of craft beers coming from Basilicata were detected, using a HS-SPME-GC-MS technique. The analysis showed the presence of various compounds (esters, alcohols, and terpenes), which were useful to determinate the aroma profiles of these beers. In addition, this profile was matched with the aroma profile of commercial beers, in order to establish the difference of the aroma profiles.

Fatty Acid Synthase as Interacting Anticancer Target of the Terpenoid Myrianthic Acid Disclosed by MS-Based Proteomics Approaches.

This research focuses on the target deconvolution of the natural compound myrianthic acid, a triterpenoid characterized by an ursane skeleton isolated from the roots of and from Nutt. (Onagraceae), using MS-based chemical proteomic techniques. Application of drug affinity responsive target stability (DARTS) and targeted-limited proteolysis coupled to mass spectrometry (t-LiP-MS) led to the identification of the enzyme fatty acid synthase (FAS) as an interesting macromolecular counterpart of myrianthic acid.

Investigational thymic stromal lymphopoietin inhibitors for the treatment of asthma: a systematic review.

Introduction: Severe asthma patients often remain uncontrolled despite high-intensity therapies. Biological therapies targeting thymic stromal lymphopoietin (TSLP), a key player in asthma pathogenesis, have emerged as potential options. Currently, the only TSLP inhibitor approved for the treatment of severe asthma is the immunoglobulin G (IgG) 2λ anti-TSLP monoclonal antibody (mAb) tezepelumab.

Artificial intelligence and pelvic fracture diagnosis on X-rays: a preliminary study on performance, workflow integration and radiologists' feedback assessment in a spoke emergency hospital.

Purpose: The aim of our study is to evaluate artificial intelligence (AI) support in pelvic fracture diagnosis on X-rays, focusing on performance, workflow integration and radiologists' feedback in a spoke emergency hospital.

Materials And Methods: Between August and November 2021, a total of 235 sites of fracture or suspected fracture were evaluated and enrolled in the prospective study. Radiologist's specificity, sensibility accuracy, positive and negative predictive values were compared to AI.

Enhanced Molecular Networking Shows sp. V1 as a Factory of Antioxidant Proline-Rich Peptides.

Two linear proline-rich peptides (-), bearing an N-terminal pyroglutamate, were isolated from the marine bacterium sp. V1, associated with the marine sponge , collected in the volcanic CO vents in Ischia Island (South Italy). Peptide production was triggered at low temperature following the one strain many compounds (OSMAC) method.

HS-SPME-GC-MS determination of the scent of taxa (fam. Orchidaceae) from Basilicata (Southern Italy).

The scent of species has been analyzed by using HS-SPME-GC-MS. The sample was collected in Basilicata (Southern Italy). Every species showed a different composition of the scent in the analyses we performed.

A potential host and virus targeting tool against COVID-19: Chemical characterization, antiviral, cytoprotective, antioxidant, respiratory smooth muscle relaxant effects of Paulownia tomentosa Steud.

COronaVIrus Disease 2019 (COVID-19) is a newly emerging infectious disease that spread across the world, caused by the novel coronavirus Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2). Despite the advancements in science that led to the creation of the vaccine, there is still an urgent need for new antiviral drugs effective against SARS-CoV-2. This study aimed to investigate the antiviral effect of Paulownia tomentosa Steud extract against SARS-CoV-2 and to evaluate its antioxidant properties, including respiratory smooth muscle relaxant effects.

The current paradigm for biologic initiation: a confirmatory quantitative analysis of self-injection training practices.

Background: Self-injected biologic therapies have gained significant prevalence across numerous therapeutic areas. A lack of specific guidance on best practices may lead to inadequate biologic initiation and training. We previously conducted a small-sample, qualitative analysis designed to identify gaps in self-injection training.

Volatile organic compounds from growing in Basilicata (Southern Italy).

SPME-GCMS analysis of vesicles of Sanguinea cultivar showed the presence of limonene (45.63%), sabinene (22.65%), and bicyclogermacrene (10.

Composition and seasonal variation of volatile organic compounds in (Lacaita) Marchi & D'Amato found at Acquapendente (Viterbo, Central Italy).

The main component of the essential oil of is viridiflorol (23.98-42.23%).

Dihydroauroglaucin Isolated from the Mediterranean Sponge Endophyte Marine Fungus Inhibits Migration of Human Neuroblastoma Cells.

Cancer cell migration is a hallmark of the aggressiveness and progression of malignancies such as high-risk neuroblastoma. Given the lack of effective therapeutic solutions to counteract cancer progression, basic research aims to identify novel bioactive molecules with inhibitory potential on cancer cell migration. In this context, this work investigated the role of members of the salicylaldehyde secondary metabolite set from the sponge endophyte fungus MUT 2316 as potential inhibitors of human neuroblastoma SH-SY5Y cell migration.

Synergism of a Novel 1,2,4-oxadiazole-containing Derivative with Oxacillin against Methicillin-Resistant .

is an important opportunistic pathogen that causes many infections in humans and animals. The inappropriate use of antibiotics has favored the diffusion of methicillin-resistant MRSA), nullifying the efforts undertaken in the discovery of antimicrobial agents. Oxadiazole heterocycles represent privileged scaffolds for the development of new drugs because of their unique bioisosteric properties, easy synthesis, and therapeutic potential.

Structure-based screening for the discovery of 1,2,4-oxadiazoles as promising hits for the development of new anti-inflammatory agents interfering with eicosanoid biosynthesis pathways.

The multiple inhibition of biological targets involved in pro-inflammatory eicosanoid biosynthesis represents an innovative strategy for treating inflammatory disorders in light of higher efficacy and safety. Herein, following a multidisciplinary protocol involving virtual combinatorial screening, chemical synthesis, and in vitro and in vivo validation of the biological activities, we report the identification of 1,2,4-oxadiazole-based eicosanoid biosynthesis multi-target inhibitors. The multidisciplinary scientific approach led to the identification of three 1,2,4-oxadiazole hits (compounds 1, 2 and 5), all endowed with IC values in the low micromolar range, acting as 5-lipoxygenase-activating protein (FLAP) antagonists (compounds 1 and 2), and as a multi-target inhibitor (compound 5) of arachidonic acid cascade enzymes, namely cyclooxygenase-1 (COX-1), 5-lipoxygenase (5-LO) and microsomal prostaglandin E synthase-1 (mPGES-1).

On the photochemical reaction of pyridinium salts with nucleophiles.

DFT calculations on the photochemical reaction of 1-n-N-butylpyridinium salt in water with hydroxide anion is in agreement with a singlet state process where the S state at λ = 253 nm can be converted into a Dewar isomer (2-butyl-2-azabicyclo[2.2.0]hexa-2,5-dien-2-yl cation).

Hydrogen atom transfer in the photochemical isomerization of hydrazones of 1,2,4-oxadiazole derivatives.

DFT calculations on the photoisomerization of hydrazones of 1,2,4-oxadiazole derivatives to 1,2,5-triazoles have been performed showing that the reaction occurred through the first excited singlet state. The Z isomer gave the reaction through a hydrogen atom transfer of the hydrazonic nitrogen atom to the nitrogen atom in four position on the oxadiazole ring. In this case, the isomerization was a concerted reaction.

The current paradigm for biologic initiation: a mixed-methods exploration of practices, unmet needs, and innovation opportunities in self-injection training.

Background: Self-injection, particularly of biologics, has become a mainstay of chronic disease management. Despite labeling requirement for healthcare provider (HCP) training, current injection initiation experiences have been shown to be suboptimal. This study characterizes gaps in training and support during initiation and identifies rationales to inform solutions.

The scent of orchids from Basilicata (Southern Italy).

The scent of species has been performed by using solid-phase microextraction- gas chromatography- mass spectrometry (SPME-GC-MS). The scent of showed the presence of 9-tricosene, 1-nonadecanol and heinecosane. The analysis of the scent of gave α-ionone as the main component.

Reinforced Smart Foams Produced with Time-Profiled Magnetic Fields.

Polymeric smart foams are lightweight and multifunctional porous materials that are sensitive to the magnetic field due to the presence of magnetic particles embedded in the matrix. Recently, a constant magnetic field has been exploited to align the particles along the magnetic field lines during the formation of the porous structure. In this paper, a new field-structuring process was developed that makes use of a time-profiled magnetic field during the foaming process to control the geometrical features of the particles aggregates.

Characterization of the volatile fraction of mastic oil and mastic gum.

GC-MS of ethyl acetate solutions and HS-SPME-GC-MS of mastic oil and mastic gum showed the presence of α-pinene and β-myrcene as main components of the mixture. In the GC-MS analysis of mastic oil 19 compounds were detected, while mastic gum allowed to detect only twelve compounds. Mastic gum showed the presence of compounds with a higher molecular weight.

Fragrance components of and collected at several sites in Italy and Germany.

The SPME-GC-MS analysis of the aroma components of subsp. , subsp. , var.

Molecular Network and Culture Media Variation Reveal a Complex Metabolic Profile in cf. D2 Associated with an Acidified Marine Sponge.

The Gram-negative D2 was isolated from the marine sponge . Sponges were collected in a shallow volcanic vents system in Ischia island (South Italy), influenced by CO emissions and lowered pH. The chemical diversity of the secondary metabolites produced by this strain, under different culture conditions, was explored by a combined approach including molecular networking, pure compound isolation and NMR spectroscopy.

Characterization of a New Mixture of Mono-Rhamnolipids Produced by Isolated from Edmonson Point (Antarctica).

Rhamnolipids (RLs) are surface-active molecules mainly produced by Antarctica is one of the less explored places on Earth and bioprospecting for novel RL producer strains represents a promising strategy for the discovery of novel structures. In the present study, 34 cultivable bacteria isolated from Edmonson Point Lake, Ross Sea, Antarctica were subjected to preliminary screening for the biosurfactant activity. The positive strains were identified by 16S rRNA gene sequencing and the produced RLs were characterized by liquid chromatography coupled to high resolution mass spectrometry (LC-HRESIMS) and liquid chromatography coupled with tandem spectrometry (LC-MS/MS), resulting in a new mixture of 17 different RL congeners, with six previously undescribed RLs.

Genomics-Metabolomics Profiling Disclosed Marine 3.6 as a Producer of a New Branched Side Chain Prodigiosin.

A wide range of prescreening tests for antimicrobial activity of 59 bacterial isolates from sediments of Ria Formosa Lagoon (Algarve, Portugal) disclosed 3.6 as the most active antibacterial producing strain. This bacterial strain, which has not previously been submitted for chemical profiling, was subjected to whole genome sequencing, which aided in the discovery and elucidation of a prodigiosin biosynthetic gene cluster that was predicted by the bioinformatic tool KEGG BlastKoala.

The composition of the aroma of orchids in Basilicata (Southern Italy).

The SPME-GC-MS analysis of the aroma components of subsp. and were reported. The relevant components of the scent of were α-amorphene, ethyl oleate, heneicosane, heptadecane, 2-heptadecanone, ethyl elaidate, pentadecane, octadecane, farnesylacetaldehyde, nonadecane, and ethyl stearate.