Susceptibility loci and chromosomal abnormalities in radiation induced hematopoietic neoplasms in mice.

Genetics of susceptibility to radiation-induced hematopoietic neoplasms and somatic chromosomal aberrations were analyzed in 305 backcross (CcS-17xCcS-2)xCcS-2 mice of two CcS/Dem recombinant congenic strains. Irradiated CcS-2 mice were previously shown to exhibit high frequency of myeloid neoplasms whereas irradiated CcS-17 mice were susceptible to T-cell lymphomas. Mice were exposed to four whole-body irradiation doses of 1.

Opposite effects of modifiers of the ApcMin mutation in intestine and mammary gland.

Patterns of tumor susceptibility in different organs are widely divergent in mouse strains: one strain may be highly susceptible to tumors in one organ but resistant in another organ, whereas another strain may exhibit the opposite pattern (P. Demant, Semin. Cancer Biol.

Genetics of susceptibility to radiation-induced lymphomas, leukemias and lung tumors studied in recombinant congenic strains.

The genetic control of susceptibility to radiation-induced tumors in mice has been tested using the series of 20 CcS/Dem (CcS) Recombinant Congenic Strains, each carrying a different random set of 12.5% of genes of the resistant strain STS/A (STS) on the genetic background of the susceptible strain BALB/cHeA (BALB/c). Two classes of tumors were frequently observed: tumors of the haematopoietic system (lymphomas, myelocytic leukemias) and lung tumors.

Effects of thiol compounds on methotrexate uptake by murine lymphocytes from thymus and thymic lymphosarcoma.

The characteristics of methotrexate (MTX) uptake and the effects of exogenous sulfhydryl compounds (i.e. reduced glutathione and cysteine) on MTX accumulation in thymocytes and thymic lymphosarcoma cells has been studied.

Effects of riboflavin on benzo(a)pyrene, 2-acetylaminofluorene and methyl methanesulfonate mutagenicity in vitro.

Riboflavin was shown to inhibit mutagenicity of benzo(a)pyrene and 2-acetylaminofluorene in the presence of S9 liver fractions deriving from B10.A mice as well as from DBA/2 mice and had no influence on mutagenicity of methyl methanesulfonate. The above findings confirm the supposition that antimutagenicity of riboflavin results from its interaction with enzymes responsible for metabolic activation of promutagens.

Cytochemical characterization of mouse L1210 leukemia.

Mouse lymphatic leukemia L1210 cells were characterized with polyclonal and monoclonal antibodies and lectins. The cells were found to have the phenotype IgG-, Thy-1.2-, Lyt-1-, Lyt-2-, asialo-GM1-, TL-, I-Ad-, IL-2R+, peanut agglutinin+, and Helix pomatia lectin +/-.

Biological characterization of three new spontaneous mouse lymphomas and comparison with the leukemia P388.

Three spontaneous lymphomas of 23- to 24-month-old (AB/Jena X DBA/2 Jena)F1 females were serially transplanted into syngeneic hosts. They were histologically classified as malignant lymphoblastic lymphomas (ABDt2 and ABDt5) and as a malignant lymphoma of histiocytic type (ABDt6). All lymphomas disseminated into the liver, spleen, and pancreas, whereas ABDt2 and ABDt5 additionally infiltrated the bone marrow and leptomeninx.

The effect of sodium-2-mercaptoethane-sulphonate, N-acetylcysteine and cysteine on the uptake of cytosine arabinoside by normal and neoplastic thymus cells of mice.

The effect of some sulfhydryl compounds on the uptake of cytosine arabinoside (Ara-C) by normal and neoplastically transformed mouse thymus cells was studied. Sodium 2-mercaptoethanesulphonate (Mesna) was found to greatly inhibit (in 80%) the uptake of Ara-C by normal cells. Two other SH-compounds (cysteine and N-acetyl-cysteine) displayed no such effect.

Evaluation of the metabolizing capacity of S9 liver preparations from rats and mice using a test with Salmonella typhimurium TA100.

The S9 phenobarbital-induced preparations from Albino rats and 6 strains of inbred and outbred Pzh: SFISS mice were tested by an Ames test for their ability to metabolize the two promutagens 2-aminofluorene (2AF) and cyclophosphamide (CP), and to influence the mutagenic activity of the two directly acting mutagens methyl methanesulphonate (MMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). 2AF showed a mutagenic activity after incubation with all kinds of microsomal preparations. S9 from B10.

Genetic monitoring of inbred mouse strains maintained at the Bhabha Atomic Research Centre, Bombay: serological typing for H-2 haplotypes and lymphocyte differentiation markers.

Seven inbred mouse strains, AKR, CBA, C3H, C57BL/6, C57BR/cd, DBA/2 and Swiss, maintained at the Bhabha Atomic Research Centre, Bombay (designated Bh) were monitored for compatibility with standard strains as well as for genetical homogeneity. For this purpose, five individual mice from each strain were typed serologically for H-2 class I and class II antigens and for lymphocyte differentiation markers, Thy-2, Lyt-1, Lyt-2, TL and Ly-10. In this latter testing, 15 inbred strains from the Institute of Immunology and Experimental Therapy, Wrocław (designated Iiw) were included.

Laboratory animals in genetical research.

The article focuses on the inbred strains of mice and their specialized derivatives in the context of genetic and developmental problems, and constitutional diseases.

H-2 antigenic specificities controlled by the translocation chromosome T190.

The H-2 antigens determined by the T(1,17)190Ca translocation chromosome were analyzed serologically. It is shown that this chromosome determines previously unknown specificities H-2.76, H-2.

Change of H-2 antigens' expression on mouse leukaemia LBN/a-2 and LBN/b-3 cells in the course of serial transplantation.

The inbred mouse strains BN/a and BN/b have haplotype H-2bp characterized by H-2.33 and lacking any other private specificity known in inbred strains. Two transplantable B cell leukaemias which originated in BN/a and BN/b mice treated with anti-lymphocyte globulin were tested serologically for H-2 antigens.

Pathologic changes in the lungs of mice following injection of human albumin microspheres.

Mice injected with 131I-human albumin microspheres equivalent to 10-20 or 200 human doses were sequentially killed over a 12-day period. About 90% of the microspheres initially lodged in the precapillary arterioles and capillaries of the lungs. Their pulmonary clearance was essentially complete after 3 days.

Leukaemias in BN/a and BN/b mice after prolonged treatment with antilymphocyte globulin.

The influence of prolonged treatment with antilymphocyte globulin on tumour incidence in BN/a and BN/b mice was studied. One ALG sample appeared to be highly leukaemogenic in two independent experiments. All treated mice developed, after a short latency period, non-thymic lymphomas with involvement of abdominal lymph nodes and spleen resulting in ascites.

Use of antilymphocyte globulin for leukemia induction in mice.

Biological activity of rabbit antimouse lymphocyte sera (ALS) and their IgG fractions was studied. Several ALS and ALG pools were cytotoxic in vitro for normal and leukemic mouse lymphocytes, causing temporary depletion of circulating leukocytes, prolongation of skin allografts, and progressive growth of L-1210/V leukemia transplanted across an H-2 barrier. As a rule, splenomegaly and a significant increase in the number of spleen cells were observed after i.