Mode of action of silver-based perovskite against Gram-negative bacteria.

Although silver is known for its antibacterial activity, its exact mode of action remains unclear. In our previous work, we described AgNbO nanoparticles (AgNbO NPs) prepared using a ceramic method, followed by high-energy and low-energy ball-milling processes, which exhibited antimicrobial activity with negligible release of Ag in deionized water. Here, we investigated thoroughly the mode of action of these AgNbO NPs against .

Growth dynamics of Escherichia coli cells on a surface having AgNbO3 antimicrobial particles.

The morphological dynamics of microbial cell proliferation on an antimicrobial surface at an early growth stage was studied with Escherichia coli on the surface of a gel supplied with AgNbO3 antimicrobial particles. We demonstrated an inhibitory surface concentration, analogous to minimum inhibitory concentration, beyond which the growth of colonies and formation of biofilm are inhibited. In contrast, at lower concentrations of particles, after a lag time the cells circumvent the antimicrobial activity of the particles and grow with a rate similar to the case in the absence of particles.

Long-Term Prevention of Arthroplasty Infections via Incorporation of Activated AgNbO Nanoparticles in PMMA Bone Cement.

We investigated the possibility of loading PMMA bone cement with antimicrobial nanostructured AgNbO particles to counter biofilm formation at the cement-tissue interface. We found that a formulation containing (1-4)% AgNbO showed high antibacterial activity against Gram-positive and Gram-negative while not showing any toxicity against THP1 human cell lines. In addition, loading the particles did not impact the mechanical properties of the cement.