Purification of a lectin from crude extract seed by a single step PEG/phosphate aqueous two-phase system.

The partitioning and purification of lectins from the crude extract of seeds (Cramoll 1,4) was investigated in aqueous two-phase systems (ATPS). A factorial design model (2) was used to evaluate the influence of polyethylene glycol (PEG) molar mass (1500-8000 g/mol), PEG concentration (12.5-17.