Functional differences in human neutrophils isolated pre- and post-prandially.

Activated polymorphonuclear leukocytes have been associated with neoplasia, atherogenesis and reperfusion injury. Since some of these conditions are also correlated with dietary fat, we examined the functional characteristics of leukocytes isolated from subjects before and after consumption of a lipid-rich meal. There was up to 2-fold greater superoxide generation in response to agonists in leukocytes obtained post-prandially; the maximum increase was observed about 4 h after eating and followed the peak (2-4 h) in serum triglycerides.

Antibody-dependent alternate pathway of complement activation in opsonophagocytosis of Porphyromonas gingivalis.

It has been suggested that the ability of Porphyromonas gingivalis to proteolyse complement, as well as its production of a capsule, contributes to resistance to phagocytosis by polymorphonuclear leukocytes. In this report, the opsonic role of serum complement and its activation pathways were investigated, using individual sera heat treated or depleted of factors B, C2, and C1q and the divalent cations Mg2+ and Ca2+. A fluorochrome microassay was used to quantitate phagocytosis of P.

Phagocytosis of virulent Porphyromonas gingivalis by human polymorphonuclear leukocytes requires specific immunoglobulin G.

No studies to date clearly define the interactions between Porphyromonas gingivalis and human peripheral blood polymorphonuclear leukocytes (PMN), nor has a protective role for antibody to P. gingivalis been defined. Using a fluorochrome phagocytosis microassay, we investigated PMN phagocytosis and killing of P.

Defective neutrophil function in an insulin-dependent diabetes mellitus patients. A case report.

The objective of this study was to evaluate the polymorphonuclear leukocyte (PMN) function in a poorly controlled adult insulin-dependent diabetic patient (IDDM) with severe recurrent periodontitis, while describing the microbiological and clinical findings. Chemotaxis, superoxide production, and phagocytosis and killing of Porphyromonas (Bacteroides) gingivalis by the IDDM PMN were evaluated 1 week before treatment relative to a healthy, matched control. These analyses revealed a significant (P less than .

A novel mouse model to study the virulence of and host response to Porphyromonas (Bacteroides) gingivalis.

We describe here the development of a mouse subcutaneous chamber model that allows for the examination of host-parasite interactions as well as the determination of gross pathology with Porphyromonas (Bacteroides) gingivalis challenge. When inoculated into stainless-steel chambers implanted subcutaneously in female BALB/c mice, P. gingivalis W83, W50, and A7436 (10(8) to 10(10) CFU) caused cachexia, ruffling, general erythema and phlegmonous, ulcerated, necrotic lesions, and death.

Deconstructing the DSM-III.

Deconstruction is a form of analysis that accounts for the unconscious elements in all human constructions, especially linguistic constructions. The theory derives its tenets from psychoanalysis and linguistics. Because the third edition of the American Psychiatric Association's Diagnostic and Statistical Manual of Mental Disorders (DSM-III) facilitates communication among practitioners of differing theoretical backgrounds, deconstruction can be used to analyze the DSM-III.

Consultative geriatric assessment for ambulatory patients. A randomized trial in a health maintenance organization.

Previous studies have shown that comprehensive geriatric assessment and follow-up can improve the health of hospitalized elderly patients. To evaluate the effectiveness of consultative geriatric assessment and limited follow-up for ambulatory patients, we randomized 600 elderly patients who were enrolled in a health maintenance organization into three groups: (1) consultation by a geriatric assessment team, (2) consultation by a "second opinion" internist, and (3) only traditional health maintenance organization services (control patients). The geriatric assessment team identified previously unrecognized problems in 35% of patients and advised changes in medication regimens for more than 40%.

The effects of monensin on the abundance of mRNA(alpha) and of sodium pumps in human cultured cells.

HeLa or MRC5-VI cells were grown for up to 1 day in media containing monensin at concentrations up to 10 microM. We measured the sodium pump density of the plasma membrane with [3H]ouabain and the mRNA for the alpha-subunit of the pump by hybridization to a cDNA probe. The sodium and potassium concentrations were measured under similar conditions, and in some experiments the rate of internalization of the sodium pumps estimated by using [3H]ouabain uptake into the cell.

Glucose transport: locus of muscle insulin resistance in obese Zucker rats.

The purposes of this study were to determine whether the muscle insulin resistance of the obese rat is due to a defect in the glucose transport process and whether the insulin resistance is fiber-type specific. The hindlimbs of fasted, 14-wk-old obese (fa/fa) and lean (fa/?) Zucker rats were perfused with perfusate containing 8 mM glucose and no insulin or 8 mM glucose and either a physiological (0.15 mU/ml), a submaximal (1.

Muscle glycogen synthesis after exercise: effect of time of carbohydrate ingestion.

The time of ingestion of a carbohydrate supplement on muscle glycogen storage postexercise was examined. Twelve male cyclists exercised continuously for 70 min on a cycle ergometer at 68% VO2max, interrupted by six 2-min intervals at 88% VO2max, on two separate occasions. A 25% carbohydrate solution (2 g/kg body wt) was ingested immediately postexercise (P-EX) or 2 h postexercise (2P-EX).

Calculation of metabolic expenditure and substrate utilization from gas exchange measurements.

At least nine different equations have been published for calculating metabolic expenditure by indirect calorimetry. This study examined the differences between equations when they are used for the nutritional assessment in an intensive care unit (ICU). Oxygen consumption and carbon dioxide production were measured in 36 ICU patients and used to calculate metabolic expenditure with the nine equations.

Outpatient geriatric assessment in a Health Maintenance Organization. Its structure, practice, and clinical implications.

The authors review their experience with the implementation of a geriatric assessment unit (GAU) within the Rhode Island Group Health Association, a Health Maintenance Organization (HMO). Included is a historical background of the involvement of HMOs with care of Medicare patients, a description of the structure of an outpatient GAU, and the authors' experience to date with this population of patients. This article concludes with a discussion of the future role of GAUs and HMOs in the care of older patients.

Exercise and diet reduce muscle insulin resistance in obese Zucker rat.

Two treatments that increase skeletal muscle insulin action are exercise training and high-carbohydrate diet. The purpose of the present study was to determine whether exercise training and a diet high in carbohydrates could function synergistically to reduce the muscle insulin resistance in the obese Zucker rat. Obese rats 4 wk of age were randomly assigned to an exercise or sedentary group.

Insulin and insulin-like growth factor (somatomedin) receptors on cloned rat pituitary tumor cells.

Specific receptors for insulin and the somatomedin peptides insulin-like growth factors I and II (IGF-I and IGF-II) have been characterized on three separate cloned strains of rat pituitary tumor cells (GH3, GH1, and GC). Binding of 125I-labeled peptides was time, temperature, and pH dependent for all three cell lines. Specific binding of [125I]insulin, which was extremely low in normal rat adenohypophyseal cells, was demonstrable for all three lines, with the Kd for the high affinity receptor ranging from 10(-10) to 4 X 10(-10) M/liter.

Single-mode-fiber saturable absorber.

Saturable absorption is performed on a signal in a single-mode fiber by allowing the evanescent field of the guided wave to interact with a saturable-absorbing dye. The evanescent field is exposed by mechanically removing a portion of the fiber cladding. The saturable material is then placed in close proximity to the fiber core.

AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress.

AppppA , ApppGpp , AppppG , ApppG , and ApppA rapidly accumulate to high levels in Salmonella typhimurium following exposure to a variety of oxidizing agents, but not to a variety of other stresses. Among the agents inducing these adenylylated nucleotides are 1-chloro-2,4-dinitrobenzene, diamide, hydrogen peroxide, t-butyl hydroperoxide, N-ethyl maleimide, iodoacetamide, cadmium chloride, and a variety of quinones. Some of these oxidizing agents cause preferential synthesis of specific adenylylated nucleotides, e.

Instrumentation for monitoring gas exchange and metabolic rate in critically ill patients.

In the critically ill patient the monitoring of oxygen consumption (VO2) and carbon dioxide production (VCO2) can identify abnormalities in tissue perfusion and metabolism. A patient's metabolic utilization can be calculated by indirect calorimetry, once VO2 and VCO2 are measured. This paper evaluates a compact instrument designed for monitoring VO2 and VCO2 in the critically ill adult.

Hospitalized cases of nonorganic failure to thrive: the scope of the problem and short-term lay health visitor intervention.

This paper describes the characteristics of thriving and failure to thrive (FTT) children and their mothers and examines the effect of short-term lay health visitor intervention in cases of nonorganic failure to thrive (NO FTT). Twenty-five FTT children and mothers received lay health visitor (LHV) intervention in addition to other community and medical treatment; 25 other FTT children and mothers did not receive the LHV intervention but did receive all other medical and community treatment. Twenty-five thriving children and mothers were matched with the FTT children and mothers in the LHV group on the child's age at intake, sex, birth weight, and the mother's age, ethnicity, and number of living children.

Non-organic failure to thrive: implications of placement through analysis of videotaped interactions.

This paper presents 6-month follow-up on a group of 16 infants hospitalized during the first months of life for non-organic failure to thrive (N-O FTT) and their mothers. Eight of these infants were placed in foster care and eight were returned home with their parents following hospitalization. The effects of placement on infant outcomes were examined through comparison of developmental scores and weight percentile changes, and the implications of foster care placements for mother-infant interactions were examined through analysis of patterns of interaction in videotaped sessions of feeding and play six months after hospital discharge.

Non-organic failure to thrive: decision for placement and videotaped evaluations.

Infants hospitalized for non-organic failure to thrive in the first six months of life are in a life-threatening situation and are already at risk for poor bonding with mother. In light of this, the meaning and use of foster care and how this separation affects the developing mother-child relationship are the issues addressed through examination of 16 cases in which 8 of the infants were placed in foster care and 8 discharged home after the failure to thrive hospitalization. This paper examines (1) maternal histories of pregnancy, labor and delivery and the neonatal status of placed and non-placed infants; (2) the developmental and weight status of placed children; (3) the nature of the decision criteria for after-hospital care; and (4) the mother-infant relationship at initial intake in terms of mother's report of events and observations of feeding and play interactions during a videotaped assessment process.

Source statistics and the Kerr effect in fiber-optic gyroscopes.

We show theoretically that polarized thermal light as generated by a superluminescent diode has the properties required to compensate for Kerr-effect drift in fiber gyroscopes. Light from some multimode lasers has related properties that can substantially reduce Kerr-effect errors. An experimental gyroscope using a multimode laser diode demonstrates no Kerr-effect error and provides significantly improved long-term stability.

Blood-gas analyzer calibration and quality control using a precision gas-mixing instrument.

We describe a new instrument that performs on-site mixing of oxygen (O2), carbon dioxide (CO2), and nitrogen (N2) to create compositions that can replace gases from standard premixed cylinders. This instrument yields accurate and predictable gas mixtures that can be used for two-point gas calibration of blood gas/pH analyzers or for liquid tonometry of either an aqueous buffer or blood used as quality-control material on blood-gas electrodes. The desired mixture of O2, CO2, and N2 is produced by microprocessor control of the sequential open-times on three solenoid valves that meter these pure gases through a common small-bore orifice.