Selection markers for transformation of the sequenced reference monokaryon Okayama 7/#130 and homokaryon AmutBmut of .

Background: Two reference strains have been sequenced from the mushroom , monokaryon Okayama 7/#130 (OK130) and the self-compatible homokaryon AmutBmut. An adenine-auxotrophy in OK130 () and a -aminobenzoic acid (PABA)-auxotrophy in AmutBmut () offer selection markers for transformations. Of these two strains, homokaryon AmutBmut had been transformed before to PABA-prototrophy and with the bacterial hygromycin resistance marker , respectively.

Growth performance and feed utilization of keureling fish (Cyprinidae) fed formulated diet supplemented with enhanced probiotic.

The objective of the present study was to determine the optimum dosage of probiotic in the diet of keureling fish ( ) fry. from Yakult® was used as a starter, and enhanced with , and molasses. The mixture was fermented for 7 days prior to use as probiotic in a formulated diet containing 30% crude protein.