Regulation of hexokinase II and glycogen synthase mRNA, protein, and activity in human muscle.

Insulin regulates the activity of key enzymes of glucose metabolism in skeletal muscle by altering transcription or translation or by producing activity-altering modifications of preexisting enzyme molecules. Because of the small size of percutaneous muscle biopsies, these phenomena have been difficult to study in humans. This study was performed to determine how physiological hyperinsulinemia regulates the activities of hexokinase (HK), glycogen synthase (GS), and GLUT-4 in human skeletal muscle in vivo.

Safety and efficacy of normalizing fasting glucose with bedtime NPH insulin alone in NIDDM.

Objective: To examine the safety and overall clinical effects of normalizing the fasting plasma glucose (FPG) level with bedtime NPH insulin alone in patients with non-insulin-dependent diabetes mellitus (NIDDM) that is poorly controlled with maximal doses of sulfonylureas.

Research Design And Methods: Twelve obese male NIDDM subjects were treated for 16 weeks with bedtime insulin after a 4-week sulfonylurea washout. The insulin dosage was increased until the FPG level was normalized.

Value of sensitive thyrotropin measurement in ambulatory and hospitalized patients.

To compare the frequency and causes of abnormal thyrotropin (TSH) levels in ambulatory and hospitalized patients and to assess the specificity and sensitivity of suppressed TSH for the diagnosis of hyperthyroidism in these two settings, analysis of thyroid function tests including sensitive TSH measurement was performed on 715 consecutive patients who had a thyroid panel performed in one clinical laboratory. Suppressed TSH (less than 0.3 mU/L) and elevated TSH (greater than 5.