A method for separating tonoplast from wheat.

Vacuoles account for 90% of plant cell volume and play important roles in maintaining osmotic pressure, storing metabolites and lysosomes, compartmentalizing harmful ions, and storing and reusing minerals. These functions closely relay on the ion channels and transporters located on the tonoplast. The separation of intact vacuoles from plant cells is the key technology utilized in the study of tonoplast-located ion channels and transporters.

Diagnosis and management of congenital type D esophageal atresia.

This study was performed to describe the current clinical practice and outcomes of type D esophageal atresia. We retrospectively analyzed 10 patients who were diagnosed with type D esophageal atresia and underwent esophageal atresia and tracheoesophageal fistula repair in the Capital Institute of Pediatrics and Beijing Children's Hospital from January 2017 to May 2022. Ten patients include three newborns and seven non-newborns.

Multiple ALMT subunits combine to form functional anion channels: A case study for rice ALMT7.

The Aluminum Activated Malate Transporter (ALMT) family members are anion channels that play important roles in organic acid transport, stress resistance, growth, development, fertilization and GABA responses. The rice malate permeable OsALMT7 influences panicle development and grain yield. A truncated mutant, ( lacking at least 2 transmembrane helices, mediates reduced malate efflux resulting in yield reducing.

Genome-Wide Analysis of the RAV Gene Family in Wheat and Functional Identification of TaRAV1 in Salt Stress.

RAV transcription factors (TFs) are unique to higher plants and contain both B3 and APETALA2 (AP2) DNA binding domains. Although sets of RAV genes have been identified from several species, little is known about this family in wheat. In this study, 26 RAV genes were identified in the wheat genome.

MYB77 regulates high-affinity potassium uptake by promoting expression of HAK5.

In Arabidopsis, the high-affinity K transporter HAK5 is the major pathway for root K uptake when below 100 µM; HAK5 responds to Low-K (LK) stress by strongly and rapidly increasing its expression during K -deficiency. Therefore, positive regulators of HAK5 expression have the potential to improve K uptake under LK. Here, we show that mutants of the transcription factor MYB77 share a LK-induced leaf chlorosis phenotype, lower K content, and lower Rb uptake of the hak5 mutant, but not the shorter root growth, and that overexpression of MYB77 enhanced K uptake and improved tolerance to LK stress.

Genome-wide identification of expansin genes in Brachypodium distachyon and functional characterization of BdEXPA27.

Plant expansin belongs to a group of cell wall proteins and functions in plant growth and development. However, limited data are available on the contributions of expansins in Brachypodium distachyon. In the present study, a total of 38 expansins were identified in B.

Wheat AGAMOUS LIKE 6 transcription factors function in stamen development by regulating the expression of .

Previous studies have revealed the functions of rice and maize () genes and , respectively, in floral development; however, the functions of three wheat () genes are still unclear. Here, we report the main functions of wheat homoeologous genes in stamen development. In RNAi plants, stamens showed abnormality in number and morphology, and a tendency to transform into carpels.

Correction: Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development.

[This corrects the article DOI: 10.1371/journal.pgen.

Comparison of Trihelix transcription factors between wheat and Brachypodium distachyon at genome-wide.

Background: Plant Trihelix transcription factors, specifically bind to GT elements and play important roles in plant physiology and development. Wheat is a main cereal crop. Brachypodium distachyon is a close relative of wheat and has been described as a new model species for studying of grass functional genomics.

BRS1 Function in Facilitating Lateral Root Emergence in Arabidopsis.

The (BRI1 Suppressor 1) gene encodes a serine carboxypeptidase that plays a critical role in the brassinosteroid signaling pathway. However, its specific biological function remains unclear. In this study, the developmental role of BRS1 was investigated in .

Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development.

The phytohormone abscisic acid (ABA) plays important roles during seed germination and early seedling development. Here, we characterized the function of the Arabidopsis WRKY6 transcription factor in ABA signaling. The transcript of WRKY6 was repressed during seed germination and early seedling development, and induced by exogenous ABA.

Arabidopsis RAV1 transcription factor, phosphorylated by SnRK2 kinases, regulates the expression of ABI3, ABI4, and ABI5 during seed germination and early seedling development.

The phytohormone abscisic acid (ABA) modulates a number of processes during plant growth and development. In this study, the molecular mechanism of Arabidopsis RAV (Related to ABI3/VP1) transcription factor RAV1 involving ABA signaling was investigated. RAV1-underexpressing lines were more sensitive to ABA than wild-type plants during seed germination and early seedling development, whereas RAV1-overexpressing lines showed strong ABA-insensitive phenotypes.