Barbed arrow-like structure membrane with ultra-high rectification coefficient enables ultra-fast, highly-sensitive lateral-flow assay of cTnI.

Acute myocardial infarction (AMI) has become a public health disease threatening public life safety due to its high mortality. The lateral-flow assay (LFA) of a typical cardiac biomarker, troponin I (cTnI), is essential for the timely warnings of AMI. However, it is a challenge to achieve an ultra-fast and highly-sensitive assay for cTnI (hs-cTnI) using current LFA, due to the limited performance of chromatographic membranes.

Single-cell RNA sequencing reveals the heterogeneity and interactions of immune cells and Müller glia during zebrafish retina regeneration.

Inflammation plays a crucial role in the regeneration of fish and avian retinas. However, how inflammation regulates Müller glia (MG) reprogramming remains unclear. Here, we used single-cell RNA sequencing to investigate the cell heterogeneity and interactions of MG and immune cells in the regenerating zebrafish retina.

Well-Tailored Norbornene-Based Fluorinated Copolymers toward Modulating Icephobicity and Mechanical Robustness.

Well-tailored construction of icephobic surfaces with mechanical robustness and investigation of the structure-property relationships at the molecular level are highly desirable. Herein, a series of norbornene-based fluorinated polyolefin copolymers (FPOR-) with varying norbornenyl dodecafluoroheptyl ester (NDFHE) molar fractions (0-100 mol %) were well-designed and fabricated via living ring-opening metathesis polymerization (ROMP) employing NDFHE and norbornenyl pentafluorophenyl ester (NPFPE) as the soft and hard segments, respectively. The mechanical and icephobic properties of the fluorinated copolymers can be regulated by adjusting the soft NDFHE contents.

A low-voltage alternant direct current electroporation chip for ultrafast releasing the genome DNA of Helicobacter pylori bacterium.

Nucleic acid detection, as an important molecular diagnostic method, is widely used in bacterial identification, disease diagnosis. For detecting the nucleic acid of bacteria, the prerequisite is to release nucleic acids inside the bacteria. The common means to release nucleic acids is the chemical method, which involves complex processes, is time-consuming, and remains chemical inhibitors.

Microstructure-Driven Self-Transport and Convection of Water on Membrane Surface for Ultra-Fast, Highly Sensitive, Low-Cost Lateral-Flow Assays.

Lateral-flow assay (LFA) is one of the most commonly used detection technologies, in which the chromatographic membranes are currently used as the lateral-flow membrane (e.g., nitrocellulose membrane, NC Mem).

A dual-functional fluorescent probe based on kaolin nanosheets for the detection and separation of aluminum ions.

Aluminum ions (Al) are closely related to environmental protection and human health, thus the detection and separation of Al is of great significance. In this study, a dual-functional fluorescent probe for the detection and separation of Al was successfully developed by grafting fluorophore onto kaolin nanosheets. The probe has the characteristics of good dispersion without the involvement of organic solvents, excellent specificity, the low limit of detection (0.

Liquid-embedded (bio)printing of alginate-free, standalone, ultrafine, and ultrathin-walled cannular structures.

While there has been considerable success in the three-dimensional bioprinting of relatively large standalone filamentous tissues, the fabrication of solid fibers with ultrafine diameters or those cannular featuring ultrathin walls remains a particular challenge. Here, an enabling strategy for (bio)printing of solid and hollow fibers whose size ranges could be facilely adjusted across a broad spectrum, is reported, using an aqueous two-phase embedded (bio)printing approach combined with specially designed cross-linking and extrusion methods. The generation of standalone, alginate-free aqueous architectures using this aqueous two-phase strategy allowed freeform patterning of aqueous bioinks, such as those composed of gelatin methacryloyl, within the immiscible aqueous support bath of poly(ethylene oxide).

A near-infrared Fluorescent Probe Based on Dicyanisophorone for the Detection of Zinc Ions (Zn) in Water and Living Cells.

As one of the important metal ions, zinc ions (Zn) are widely involved in various physiological and pathological processes, and play fundamental roles in neurotransmission, cell metabolism and apoptosis. However, the convenient monitor of Zn in environmental and biological samples remains challenging. In this study, a small molecule dicyanoisophorone-based schiff base incorporating with o-phenylenediamine was synthesized.

The value of CT features and demographic data in the differential diagnosis of type 2 papillary renal cell carcinoma from fat-poor angiomyolipoma and oncocytoma.

Purposes: To determine the CT features and demographic data predictive of type 2 papillary renal cell carcinoma (PRCC) that can help distinguish this neoplasm from fat-poor angiomyolipoma (fpAML) and oncocytoma.

Methods: Fifty-four patients with type 2 PRCC, 48 with fpAML, and 47 with oncocytoma in the kidney from multiple centers were retrospectively reviewed. The demographic data and CT features of type 2 PRCC were analyzed and compared with those of fpAML and oncocytoma by univariate analysis and multiple logistic regression analysis to determine the predictive factors for differential diagnosis.

Morphologic CT and MRI features of primary parotid squamous cell carcinoma and its predictive factors for differential diagnosis with mucoepidermoid carcinoma.

Background: Primary parotid squamous cell carcinoma (SCC) is a rare entity with a poor prognosis. Pathologically, the diagnosis of it requires the exclusion of parotid mucoepidermoid carcinoma (MEC). Currently, the imaging features of primary parotid SCC and the predictive indicators for differential diagnosis of the two entities have not been well reported.

Micropore-Forming Gelatin Methacryloyl (GelMA) Bioink Toolbox 2.0: Designable Tunability and Adaptability for 3D Bioprinting Applications.

It is well-known that tissue engineering scaffolds that feature highly interconnected and size-adjustable micropores are oftentimes desired to promote cellular viability, motility, and functions. Unfortunately, the ability of precise control over the microporous structures within bioinks in a cytocompatible manner for applications in 3D bioprinting is generally lacking, until a method of micropore-forming bioink based on gelatin methacryloyl (GelMA) was reported recently. This bioink took advantage of the unique aqueous two-phase emulsion (ATPE) system, where poly(ethylene oxide) (PEO) droplets are utilized as the porogen.

Two near-infrared fluorescent probes based on dicyanoisfluorone for rapid monitoring of Znand Pb.

Zinc (Zn) and lead (Pb) ions in the environment have important effects on human health and environmental safety. Therefore, it is necessary to effectively detect them by a convenient and reliable analysis method. In this study, two near-infrared fluorescent probes for the fast determination of Znand Pbwere synthesized by a simple Schiff base reaction between the dicyanoisophorone skeleton and carbohydrazide derivatives.

Context-dependent effects of inflammation on retina regeneration.

Inflammation is required for the proliferation of Müller glia (MG) into multipotent progenitors (MGPCs) in the injured fish and avian retinas. However, its function in retina regeneration has not been fully understood. Here we investigated the role of inflammation in three different retinal regeneration paradigms in zebrafish (stab-injury, NMDA-injury and insulin treatment).

A near infrared fluorescent probe for detection and bioimaging of zinc ions and hypochlorous acid.

It is of great significance to monitor zinc ions (Zn) and hypochlorous acid (HClO) conveniently in disease diagnosis and environmental protection. Herein, we successfully prepared a near-infrared fluorescent probe based on dicyanoisophorone derivative and methyl hydrazate by Schiff base reaction for effective detection of Zn and HClO. After binding with Zn at a molar ratio of 1:1, the fluorescence intensity (λ = 482 nm, λ = 653 nm) of the probe was significantly enhanced, thus achieving quantitative detection and optical cell imaging of Zn.

Role of computed tomography features in the differential diagnosis of chromophobe renal cell carcinoma from oncocytoma and angiomyolipoma without visible fat.

Background: Chromophobe renal cell carcinoma (chRCC) is often confused with oncocytoma and angiomyolipoma without visible fat (AML.wovf). The aim of this study was to determine computed tomography (CT) features predictive of chRCC to distinguish it from oncocytoma and AML.

A Critical Review on Organic Small Fluorescent Probes for Monitoring Carbon Monoxide in Biology.

Endogenous carbon monoxide (CO) is an important intracellular gas messenger that is intimately involved in many physiological and pathological processes. The abnormal concentration of CO in living organisms can cause many diseases. Therefore, it is of great significance to monitor CO in biological samples.

Uniaxial and Coaxial Vertical Embedded Extrusion Bioprinting.

The 3D bioprinting technologies have attracted increasing attention due to their flexibility in producing architecturally relevant tissue constructs. Here, a vertical embedded extrusion bioprinting strategy using uniaxial or coaxial nozzles is presented, which allows formation of vertical structures of homogeneous or heterogeneous properties. By adjusting the bioprinting parameters, the characteristics of the bioprinted vertical patterns can be precisely controlled.

Enhanced luminescence performances of BaLaMgTaO:Mn red phosphor by Bi, Ca doping for indoor plant lighting supplementary LED.

A new perovskite BaLaMgTaO:Mn (BLMTO:Mn) red phosphor was synthesized for the first time via the high-temperature solid-state method. The emission band of the phosphor ranges from 650 to 750 nm, which matches well with the absorption band of P and P. By doping of Bi and Ca ions in the BLMTO:Mn phosphor, a 4.

Prediction of fetal weight based on back propagation neural network optimized by genetic algorithm.

Fetal weight is an important index to judge fetal development and ensure the safety of pregnant women. However, fetal weight cannot be directly measured. This study proposed a prediction model of fetal weight based on genetic algorithm to optimize back propagation (GA-BP) neural network.

Bile Acids Impair Vaccine Response in Children With Biliary Atresia.

Background: Vaccination is the best way to protect children under 5 years from death or disability. Children with biliary atresia (BA), which is the most common pediatric cholestatic end-stage liver disease (PELD), are more vulnerable to infectious diseases. However, the vaccination coverage and factors modulating vaccine responses in children with BA are largely unknown.

Predictive CT features for the diagnosis of primary pulmonary mucoepidermoid carcinoma: comparison with squamous cell carcinomas and adenocarcinomas.

Background: To determine the predictive CT imaging features for diagnosis in patients with primary pulmonary mucoepidermoid carcinomas (PMECs).

Materials And Methods: CT imaging features of 37 patients with primary PMECs, 76 with squamous cell carcinomas (SCCs) and 78 with adenocarcinomas were retrospectively reviewed. The difference of CT features among the PMECs, SCCs and adenocarcinomas was analyzed using univariate analysis, followed by multinomial logistic regression and receiver operating characteristic (ROC) curve analysis.

Low Ice Adhesion Surfaces Based on Flexible Fluorinated Polymers with a Polynorbornene Backbone.

Realizing icephobic surfaces with low ice adhesion and durability continues to be fascinating as well as challenging. Herein, a norbornene-based fluorinated polymer (NFP) with high flexibility and high tensile strength is designed and fabricated using a fluorinated side chain and a norbornene backbone, displaying low ice shear strength less than 20 kPa and excellent durability. Experimental and theoretical analyses show that the flexibility of the polymer chains and the synergistic macromolecular aggregation of the fluorinated side groups and the norbornene backbone play key roles in the excellent surface icephobic properties of the NFP films.

Predictive Computed Tomography and Clinical Features for Diagnosis of COVID-19 Pneumonia: Compared With Common Viral Pneumonia.

Objective: To determine the predictive computed tomography (CT) and clinical features for diagnosis of COVID-19 pneumonia.

Methods: The CT and clinical data including were analyzed using univariate analysis and multinomial logistic regression, followed by receiver operating characteristic curve analysis.

Results: The factors including size of ground grass opacity (GGO), GGO with reticular and/or interlobular septal thickening, vascular enlargement, "tree-in-bud" opacity, centrilobular nodules, and stuffy or runny nose were associated with the 2 groups of viral pneumonia, as determined by univariate analysis (P < 0.

CT differential diagnosis of COVID-19 and non-COVID-19 in symptomatic suspects: a practical scoring method.

Background: Although typical and atypical CT image findings of COVID-19 are reported in current studies, the CT image features of COVID-19 overlap with those of viral pneumonia and other respiratory diseases. Hence, it is difficult to make an exclusive diagnosis.

Methods: Thirty confirmed cases of COVID-19 and forty-three cases of other aetiology or clinically confirmed non-COVID-19 in a general hospital were included.