New Polyketide Congeners with Antibacterial Activities from an Endophytic Fungus 17035 (China General Microbiological Culture Collection Center No. 40666).

Four new polyketides, heterocornol Y (), stemphyindan (), pestalospirane C (), and stemphyspyrane (), along with five known ones (-) were isolated from the endophytic fungus 17035 (SG17035) based on the One Strain Many Compounds (OSMAC) strategy allied with an LC-MS approach. These structures were elucidated through extensive spectroscopic analyses, single-crystal X-ray diffraction, and C NMR-DP4 analysis. Pestalospirane C () and stemphyspyrane () featured unprecedented spiroketal skeletons.

Identifying sesterterpenoids via feature-based molecular networking and small-scale fermentation.

Terpenoids are known for their diverse structures and broad bioactivities with significant potential in pharmaceutical applications. However, natural products with low yields are usually ignored in traditional chemical analysis. Feature-based molecular networking (FBMN) was developed recently to cluster compounds with similar skeletons, which can highlight trace amounts of unknown compounds.

Norditerpenoids biosynthesized by variediene synthase-associated P450 machinery along with modifications by the host cell .

The chemical diversity of terpenoids is typically established by terpene synthase-catalyzed cyclization and diversified by post-tailoring modifications. Fungal bifunctional terpene synthase (BFTS) associated P450 enzymes have shown significant catalytic potentials through the development of various new terpenoids with different biological activities. This study discovered the BFTS and its related gene cluster from the plant endophytic fungus 17020.

Anti-Food Allergic Compounds from MCCC 3A00225, a Deep-Sea-Derived Fungus.

Ten new (-) and 26 known (-) compounds were isolated from MCCC 3A00225, a deep sea-derived fungus. The structures of the new compounds were determined by detailed analysis of the NMR and HRESIMS spectroscopic data. The absolute configurations were established by X-ray crystallography, Marfey's method, and the ICD method.

Viridicatol Isolated from Deep-Sea Alleviates Anaphylaxis and Repairs the Intestinal Barrier in Mice by Suppressing Mast Cell Activation.

Viridicatol is a quinoline alkaloid isolated from the deep-sea-derived fungus The structure of viridicatol was unambiguously established by X-ray diffraction analysis. In this study, a mouse model of ovalbumin-induced food allergy and the rat basophil leukemia (RBL)-2H3 cell model were established to explore the anti-allergic properties of viridicatol. On the basis of the mouse model, we found viridicatol to alleviate the allergy symptoms; decrease the levels of specific immunoglobulin E, mast cell protease-1, histamine, and tumor necrosis factor-α; and promote the production of interleukin-10 in the serum.

Penigrisacids A-D, Four New Sesquiterpenes from the Deep-Sea-Derived .

Four new (penigrisacids A-D, -) and one known () carotane sesquiterpenoids were isolated from the deep-sea-derived fungus , along with four known compounds (-). The planar structures and relative configurations of the new compounds were determined by extensive analysis of the NMR and HRESIMS data. The absolute configurations were established by comparison of the experimental and calculated ECD (electronic circular dichroism) spectra or OR (optical rotation) value.

Cytotoxic -terphenyls from the deep-sea-derived .

From the deep-sea-derived fungus , one novel () and three known (-) -terphenyl derivates were isolated. The structure of the new compound was established mainly on the basis of extensive analysis of 1D and 2D NMR data. All four isolates were tested for anti-food allergic and antitumor bioactivities.

Sarocladione, a unique 5,10:8,9-diseco-steroid from the deep-sea-derived fungus Sarocladium kiliense.

A novel ergostane, sarocladione (1), was isolated from the deep-sea-derived fungus Sarocladium kiliense, along with 20 known compounds. The structure of 1 was determined mainly by a detailed analysis of its experimental and calculated NMR spectroscopic data. It is worth noting that 1 was the first steroid bearing a 5,10:8,9-diseco moiety.