A systematic study of Tupaia as a model for human acute hepatitis B infection.

The molecular features of hepatitis B virus (HBV) infection, eradication, and pathogenesis are poorly understood, partly due to the lack of an adequate animal model that faithfully reproduces the course of infection. Although Tupaia belangeri were previously recognized as HBV-susceptible animals, the course of infection in adult tupaias remains obscure. Herein, we performed a longitudinal study and demonstrated that adult tupaias were efficiently infected (90% infection rate) with 10 copies of the HBV genome.

Hepalatide ameliorated progression of nonalcoholic steatohepatitis in mice.

Background: No FDA-approved medications are available for the treatment of nonalcoholic steatohepatitis (NASH). The present study aimed to assess the effects of Hepalatide, a sodium taurocholate cotransporting polypeptide (NTCP) receptor-binding agent, on metabolic and histopathologic changes of a mouse model of NASH caused by high fat/calorie diet plus high fructose/glucose in drinking water (HFCD-HF/G) for 16 weeks.

Methods: Male mice were randomly divided into 4 groups: controls (normal diet), HFCD-HF/G group, HFCD-HF/G plus low or high dose of Hepalatide (20 or 60 mg/kg, LH or HH, s.

[Screening of Germination and Seedling Conditions of Trollius chinensis Seeds from Zhangjiakou].

Objective: To screen the germination and seedling conditions of Trollius chinensis seeds from Chongli county, Zhangjiakou city. To provide the theoretical basis and practical technology for establishing technology system of seedling and cultivation of Trollius chinensis seeds in Zhangjiakou city.

Methods: The data were collected and analyzed by the contrast test of greenhouse.

Two-stage cultivation of Pseudomonas sp. F12 for the production of enzymes converting DL-2-amino-Δ²-thiazoline-4-carboxylic acid to L-cysteine.

Pseudomonas sp. F12 isolated from soil could transform DL-2-amino-Δ(2)-thiazoline-4-carboxylic acid (DL-ATC) to L-cysteine. It could grow in minimal medium containing DL-ATC as the sole carbon and nitrogen source, and the apparent activity of L-cysteine synthesis (CS) achieved 122 U/mL in a 5-L bioreactor.