[Predictive value of plasma heparin-binding protein combined with albumin for 28-day mortality in patients with sepsis].

Objective: To evaluate the predictive value of plasma heparin-binding protein (HBP) combined with albumin (Alb) for predicting 28-day mortality in patients with sepsis.

Methods: The clinical data of patients with sepsis admitted to the emergency intensive care unit (EICU) of the People's Hospital of Shenzhen Baoan District from March 2020 to March 2024 were retrospectively analyzed. The study began at the time of the first diagnosis of sepsis upon EICU admission and ended upon patient death or at 28 days.

Split-aptamer mediated regenerable temperature-sensitive electrochemical biosensor for the detection of tumour exosomes.

In this paper, a split-aptamer mediated regenerable temperature-sensitive (SMRT) electrochemical biosensor was constructed for the detection of exosomes. The split-aptamer used in this SMRT biosensor was composed of two fragments, one of which was immobilized on the surface of an electrode via sulfhydryl groups and named split-a and the other was labelled with methylene blue and named split-b. The two fragments could form sandwich structures at the electrode surface via target-induced self-assembly in the presence of target exosomes at 4 °C in PBS, and then realizing the detection of exosomes via voltammetry.

Scutellarein relieves the death and inflammation of tubular epithelial cells in ischemic kidney injury by degradation of COX-2 protein.

Objectives: Acute kidney injury (AKI) is a clinical syndrome that usually caused by ischemia/reperfusion (I/R). Previous studies have revealed the protection of scutellarein against ischemia in nervous system. This study aimed to demonstrate the potential of scutellarein in ischemic AKI.

Large-scale translatome profiling annotates the functional genome and reveals the key role of genic 3' untranslated regions in translatomic variation in plants.

The translatome, a profile of the translational status of genetic information within cells, provides a new perspective on gene expression. Although many plant genomes have been sequenced, comprehensive translatomic annotations are not available for plants due to a lack of efficient translatome profiling techniques. Here, we developed a new technique termed 3' ribosome-profiling sequencing (3'Ribo-seq) for reliable, robust translatomic profiling.

A novel molecularly imprinted sensor based on PtCu bimetallic nanoparticle deposited on PSS functionalized graphene with peroxidase-like activity for selective determination of puerarin.

In this work, PtCu bimetallic nanoparticle was deposited on poly (styrene sulfonate) (PSS) functionalized graphene (Gr) to form a nanocomposite PtCu/PSS-Gr and its enzyme-like activity was investigated. Benefiting from the synergistic effect from Pt and Cu monometal as well as the superior properties of PSS-Gr, such as large surface area, good dispersity, strong adsorption of substrate and additional peroxidase-like activity, the PtCu/PSS-Gr nanocomposite was demonstrated as an excellent peroxidase mimic to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of HO. Combined with molecularly imprinted polymer (MIP), a new colorimetric approach for puerarin detection was proposed with the linear range of 2 × 10-6 × 10 mol L and LOD of 1 × 10 mol L.

Flower-like MoS decorated with CuO nanoparticles for non-enzymatic amperometric sensing of glucose.

In this study, a novel nanohybrid consisting of flower-like MoS decorated with CuO nanoparticles has been successfully synthesized for non-enzymatic amperometric sensing of glucose. Structural characterizations revealed that CuO nanoparticles were highly dispersed on MoS nanosheets. Electrochemical performances were investigated by cyclic voltammetry (CV) and chronoamperometry.

De novo comparative transcriptome analysis provides new insights into sucrose induced somatic embryogenesis in camphor tree (Cinnamomum camphora L.).

Background: Somatic embryogenesis is a notable illustration of cell totipotency, by which somatic cells undergo dedifferentiation and then differentiate into somatic embryos. Our previous work demonstrated that pretreatment of immature zygotic embryos with 0.5 M sucrose solution for 72 h efficiently induced somatic embryo initiation in camphor tree.