Publications by authors named "Cuie Guang"

Article Synopsis
  • Deoxynivalenol (DON) is a harmful mycotoxin produced by Fusarium species, commonly found in wheat and grains, and has been a focus for enzymatic degradation studies.
  • The study identifies a dehydrogenase enzyme (Pe DDH) from Pelagibacterium sp. SCN 63-126 that effectively breaks down DON, demonstrating optimal activity at 35°C and pH 8.5.
  • Pe DDH's activity is significantly enhanced by the presence of calcium, magnesium, copper, and certain chemical cofactors, allowing it to degrade DON quickly, highlighting its potential for reducing DON contamination in grains.*
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Article Synopsis
  • - Deoxynivalenol (DON) is a harmful mycotoxin produced by Fusarium fungi, commonly contaminating grains like wheat, which poses risks to health and agriculture; enzymatic treatments have emerged as effective methods to control its levels.
  • - A new enzyme called DON dehydrogenase (DeDDH) from Devosia yakushimensis was found to be most active at 35°C and pH 7.5, with good stability at higher temperatures and the ability to degrade 75% of DON within 4 hours, further enhanced by specific cofactors.
  • - Key residues in the enzyme's structure were identified as crucial for its activity, but more research is necessary to understand its structure and
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Lacto--triose II (LNTri II), an important precursor for human milk oligosaccharide (HMOs) synthesis, has garnered significant attention due to its structural features and physiological properties. Composed of galactose (Gal), -acetylglucosamine (GlcNAc), and glucose (Glc), with the chemical structure GlcNAcβ1,3Galβ1,4Glc, the distinctive structure of LNTri II confers various physiological functions such as promoting the growth of beneficial bacteria, regulating the infant immune system, and preventing certain gastrointestinal diseases. Extensive research efforts have been dedicated to elucidating efficient enzymatic synthesis pathways for LNTri II production, with particular emphasis on the transglycosylation activity of β--acetylhexosaminidases and the action of β-1,3--acetylglucosaminyltransferases.

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Patulin (PAT) is a mycotoxin commonly found in fruits and vegetables, prompting the need for effective removal and detoxification methods, which have garnered significant research attention in recent years. Among these methods, the utilization of microbial-derived enzymes stands out due to their mild operating conditions, specificity in targeted functional groups, and the production of non-toxic by-products, making it a preferred degradation approach. In this study, a novel PAT-degrading enzyme derived from Cyberlindnera fabianii (Cyfa-SDR) was identified, demonstrating its highest catalytic activity at pH 7.

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Human milk oligosaccharides (HMOs) have been recognized as gold standard for infant development. 3-Fucosyllactose (3-FL), being one of the Generally Recognized as Safe HMOs, represents a core trisaccharide within the realm of HMOs; however, it has received comparatively less attention in contrast to extensively studied 2'-fucosyllactose. The objective of this review is to comprehensively summarize the health effects of 3-FL, including its impact on gut microbiota proliferation, antimicrobial effects, immune regulation, antiviral protection, and brain maturation.

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Article Synopsis
  • 3-Fucosyllactose (3-FL) is a beneficial human milk oligosaccharide for infants, and researchers optimized a bacterial strain to enhance its production.
  • They inactivated competing genes and tested transporters to increase extracellular 3-FL output, achieving a significant doubling in production.
  • A modified version of an enzyme (FutM2-Q126A) was developed, resulting in a strain capable of producing high yields of 3-FL, demonstrating strong potential for industrial use.
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Carbohydrate-active enzymes are accountable for the synthesis and degradation of glycosidic bonds among diverse carbohydrates. Fructosyl-transferases represent a subclass of these enzymes, employing sucrose as a substrate to generate fructooligosaccharides (FOS) and fructan polymers. This category primarily includes levansucrase (LS, EC 2.

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Zearalenone (ZEN) and its derivatives are prevalent contaminants in cereal crops. This study investigated a novel thermostable ZEN lactonase (ZENM) from sp. GIB2.

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Deoxynivalenol (DON) poses a significant threat to human health due to its widespread distribution and biological toxicity. Here, we identified a novel DON-degrading enzyme from Youhaiella tibetensis (YoDDH). YoDDH exhibited the highest activity against DON at pH 4.

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Currently, people pay more attention to marine sugars, because of their unique physiological effects. Alginate oligosaccharides (AOS) are the degradation products of alginate and have been used in food, cosmetic, and medicine fields. AOS display good physical characteristics (low relative molecular weight, good solubility, high safety, and high stability) and excellent physiological functions (immunomodulatory, antioxidant, antidiabetic, and prebiotic activities).

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Flavonoids are ubiquitous and diverse in plants and inseparable from the human diet. However, in terms of human health, their further research and application in functional food and pharmaceutical industries are hindered by their low water solubility. Therefore, flavonoid glycosylation has recently attracted research attention because it can modulate the physicochemical and biochemical properties of flavonoids.

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Pyrethroids, which are synthetic organic insecticides, are widely used in agriculture and households to resist pests and control disease transmission. However, pyrethroids have inevitably caused environmental pollution, leading to concerns for food safety and human health. Bioremediation has emerged as one of the most promising methods to eliminate pyrethroids compounds.

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Zearalenone (ZEN) and its derivatives are one of the most contaminated fungal toxins worldwide, posing a severe threat to food security and human life. Traditional physical and chemical detoxifying methods are unsatisfactory due to incomplete detoxification, nutrient loss, and secondary pollutants. In recent years, bioremediation for eliminating fungal toxins has been gradually investigated.

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Carbamate pesticides are widely used in the environment, and compared with other pesticides in nature, they are easier to decompose and have less durability. However, due to the improper use of carbamate pesticides, some nontarget organisms still may be harmed. To this end, it is necessary to investigate effective removal or elimination methods for carbamate pesticides.

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Recently, the biosynthesis of human milk oligosaccharides (HMOs) has been attracting increasing attention. Lacto--neotetraose (LNnT) is one of the most important neutral-core HMOs with promising health effects for infants. It has received Generally Recognized as Safe (GRAS) status and is the second HMO commercially added in infant formula after 2'-fucosyllactose.

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Many drugs and prebiotics derive their activities from sugar substituents. Due to the prevalence and complexity of these biologically active compounds, enzymatic glycodiversification that facilitates easier access to these compounds can make profound contributions to the pharmaceutical, food, and feed industries. Amylosucrases (ASases) are attractive tools for glycodiversification because of their broad acceptor substrate specificity, but the lack of structural information and their poor thermostability limit their industrial applications.

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D-Mannose has great value in the treatment of chronic diseases. D-Mannose isomerase can catalyze the bioconversion of D-fructose to D-mannose. Therefore, a novel D-mannose isomerase gene (Strh-MIase) from Stenotrophomonas rhizophila strain IS26 was expressed, purified, and characterized for the industrial production of D-mannose.

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Phenyllactic acid (PLA) is capable of inhibiting the growth of many microorganisms, showing a broad-spectrum antimicrobial property, which allows it to hold vast applications in the: food, feed, pharmaceutical, and cosmetic industries, especially in the field of food safety. Recently, the production of PLA has garnered considerable attention due to the increasing awareness of food safety from the public. Accordingly, this review mainly updates the recent development for the production of PLA through microbial fermentation and whole-cell catalysis (expression single-, double-, and triple-enzyme) strategies.

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D-Allulose is the C-3 epimer of D-fructose, and widely regarded as a promising substitute for sucrose. It's an excellent low-calorie sweetener, with 70% sweetness of sucrose, 0.4 kcal/g dietary energy, and special physiological functions.

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In the past decades, the organophosphorus compounds had been widely used in the environment and food industries as pesticides. Owing to the life-threatening and long-lasting problems of organophosphorus insecticide (OPs), an effective detection and removal of OPs have garnered growing attention both in the scientific and practical fields in recent years. Bacterial organophosphorus hydrolases (OPHs) have been extensively studied due to their high specific activity against OPs.

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D-Galactose is an abundant carbohydrate monomer in nature and widely exists in macroalgae, plants, and dairy wastes. D-Galactose is useful as a raw material for biomass fuel production or low-calorie sweetener production, attracting increased attention. This article summarizes the studies on biotechnological processes for galactose utilization.

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Currently, low sugar and low energy have become an important trend in the food industries. Therefore, the bioconversion of the functional low-calorie rare sugars attracts more and more attention. l-Ribulose 3-epimerase (LREase) belongs to the ketose 3-epimerase (KEase) family, which could not only efficiently catalyze the reversible C-3 epimerization between l-ribulose and l-xylulose but also between d-fructose and d-allulose.

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