Correlation of DNA load, genotyping, and clinical phenotype of infection in children.

Introduction: This study aimed to investigate the correlation between (MP)DNA load in the bronchoalveolar lavage fluid (BALF) of children with MP pneumonia (MPP) and its subtypes, relevant laboratory data, imaging, extrapulmonary complications in infected children, and its clinical significance in evaluating the disease.

Methods: Children hospitalized with MPP at Tianjin Children's Hospital between December 2017 and December 2020 were selected for the study, excluding those with mixed viral, bacterial, and fungal infections. Children were divided into low- and high-load groups according to the MP DNA load in BALF using real-time quantitative fluorescence polymerase chain reaction (PCR).

Interleukin-6 serum levels are independently associated with severe adenovirus pneumonia in children: a cross-sectional study.

Background: This study aimed to explore the potential association between interleukin-6 (IL-6) serum levels and severe adenovirus pneumonia (SAP) in children.

Methods: A retrospective hospital-based cross-sectional study was conducted on children with SAP who presented to the Tianjin Children's Hospital between January 2019 and December 2020. Serum IL-6 levels were categorized into quintiles (Q1-5).

Establishment of a predictive nomogram and its validation for severe adenovirus pneumonia in children.

Background: Severe adenovirus pneumonia (SAP) of children is prone to multi-system complications, has the high mortality rate and high incidence of sequelae. Severity prediction can facilitate an adequate individualized treatment plan. Our study try to develop and evaluate a predictive nomogram for children with SAP.

Analysis of the predicting factors of recurrent wheezing in infants.

Background: Clinically, asthma in children under 5 years old is under estimated because lack of diagnostic criteria. The current study was, therefore, designed to identify the predicting factors for recurrent wheezing in infants.

Methods: One hundred forty-five infants under 3-year old hospitalized with respiratory diseases were enrolled into this study.

Phenotypes of the inflammatory cells in the induced sputum from young children or infants with recurrent wheezing.

Background: To identify inflammatory cell types by phenotypic analysis of the inflammatory cells in the induced sputum.

Methods: This retrospective study included 1232 children and infants, who were assigned into mild/moderate groups (326) and severe group (602) by clinical symptom scores. Phenotypes of sputum inflammatory cells were analyzed using liquid-based thin-cytologic test and eosinophil-derived neurotoxin (EDN) was quantified by ELISA.