Aims: A pilot survey shows that primary nocturnal enuresis (PNE) prevalence has increased significantly during the past decade in Mainland China. Whether it is related to the delay of elimination communication (EC) is unclear. This study retrospectively investigated the influence of delayed EC on the PNE prevalence in children and adolescents in mainland China.
View Article and Find Full Text PDFInvestigations were made to identify the causal agent of an acute outbreak of abortions in a domesticated herd of wild boar. Only porcine parvovirus (PPV) was isolated from samples of organs from the still-born sucklings and mummified aborted fetuses. The isolated virus hemagglutinated erythrocytes of guinea pig, murine, rat, and chicken.
View Article and Find Full Text PDFXi Bao Yu Fen Zi Mian Yi Xue Za Zhi
May 2010
Aim: To prepare monoclonal antibodies (mAb) against HN protein of Class I Newcaslte disease virus.
Methods: Several 6-week-old mice were immunized with ClassI 9a5b virulent strain. Hemagglutination inhibition (HI), indirect immunofluorescence assay (IFA), cell neutralization test, ELISA and Western blot analysis were used to characterize and identify these antibodies.
The viral protein 22 (VP22) in the tegument of Marek's disease virus serotype 1 (MDV-1) plays an important role in cell-to-cell spread and viral propagation. Antiserum against the carboxyl terminus of VP22 was prepared by immunizing mice with recombinant VP22 expressed in E. coli, and used to investigate its expression in chicken embryo fibroblast (CEF) cells infected with different MDV-1 strains.
View Article and Find Full Text PDFXi Bao Yu Fen Zi Mian Yi Xue Za Zhi
March 2007
Aim: To prepare and characterize monoclonal antibodies (mAb) against VP22 carboxyl terminus of CVI988/Rispens strain of Marek's disease virus serotype 1.
Methods: Carboxyl terminus of CVI988 VP22 (94aa-243aa) was expressed in prokaryotic system. mAbs against VP22 were prepared by hybridoma technology from BALB/c mice immunized with the fusion protein GST-VP22C and characterized by ELISA, indirect fluorescence assay (IFA) and Western blot.
Zhonghua Liu Xing Bing Xue Za Zhi
June 2006
Objective: To explore the awareness on sever acute respiratory syndrome (SARS) and public health emergencies among general publics.
Methods: A cluster sampling method was implemented in Harbin and Jiagedaqi district of Daxinanling of Heilongjiang province. Research subjects were divided into three groups as city, township and rural areas and were given questionnaires to fill in.
Studies on host responses in sheep artificially infected with Theileria sp. (China) were discussed and summarized mainly on typical high fever periods, merozoits and schizoon observation, antibody response.
View Article and Find Full Text PDFZhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
March 2005
Objective: To design and rapidly evaluate a TaqMan assay for detecting influenza A viruses.
Methods: The probe and the primers of the assay were designed with the software packages of DNA Star and Primer Premier 5.0.