Publications by authors named "Cui-Cai Zhang"
Article Synopsis
- The study investigates a smooth phage-resistant strain of cattle brucellosis, Bab8416, which shows discrepancies between traditional typing methods and modern molecular techniques.
- Whole genome sequencing reveals significant genetic differences between Bab8416 and other smooth phage-sensitive strains, suggesting evolutionary divergence.
- Findings indicate that specific genetic rearrangements and mutations in Bab8416 could serve as key factors in understanding phage resistance and may help improve taxonomic classification of the species.
Objective: To identify and type three leptospires isolated from Rattus tanezumi in Guizhou Province by using three molecular techniques (PFGE, MLVA, and MLST), reveal the molecular characteristic of causative agents of local leptospirosis and evaluate these three molecular methods based on their detection resolution and efficiency.
Methods: Three Leptospira strains were isolated from the kidney of Rattus tanezumi and cultured with EMJH medium. PFGE, MLVA, and MLST assays were applied to type the three strains isolated from Rattus tanezumi in Guizhou Province.
[Establishment and application of TaqMan Real-time PCR for the detection of pathogenic Leptospira species].
Zhonghua Liu Xing Bing Xue Za Zhi
October 2011
Objective: To develop and evaluate a TaqMan Real-time PCR method for the detection of pathogenic Leptospira species.
Methods: rrs gene of part fragment on 16S rRNA was used to design primers and TaqMan probe. The target gene was cloned into vector pMD19-T in order to make the standard curve and be used for quality control.
[Application of multilocus variable-number tandem-repeat analysis for gene typing and epidemiology of Leptospira interrogans].
Zhonghua Liu Xing Bing Xue Za Zhi
April 2008