Intestinal dysbiosis in pediatric Crohn's disease patients with mutations.

Background: Several studies have employed animal models to explore the association between microbiota and interleukin (IL) 10 signaling; however, limited information is available about the human microbiome.

Aim: To characterize the microbiome in patients with mutations and to explore the association between gut dysbiosis and disease severity.

Methods: Fecal samples were collected from patients who were diagnosed with loss-of-function mutations in the gene between January 2017 and July 2018 at the Children's Hospital of Fudan University.

Special clinical characteristics and outcomes in Chinese pediatric patients with early-onset Crohn's disease.

Objective: To study the clinical and nutritional characteristics of early-onset Crohn's disease (EO-CD) in China.

Methods: Patients were defined as having EO-CD (age at diagnosis <10 y) or late-onset Crohn's disease (LO-CD; age at diagnosis of 10-17 y). Their characteristics, clinical, and nutritional data were collected at baseline and at each follow-up visit.

A20 inhibits lipopolysaccharide-induced inflammation in enterocytes.

Aim: To examine the role of A20 in the regulation of intestinal epithelial cells (IECs) inflammation.

Methods: Using gene transfection, both stable overexpression and knockdown A20-expressed HT-29 cell lines were established. Accordingly, the cells were divided into the following groups: The control group, the A20 overexpression group, the A20 knockdown group and the respective controls.

Coordination and nursing care of pediatric patients undergoing double balloon enteroscopy.

Aim: To review safety, efficacy, and proper nursing care of double-balloon enteroscopy (DBE) in pediatric patients with small intestinal disease.

Methods: Our study included 37 patients with abdominal pain, diarrhea, passage of blood in the stools, and other symptoms, who underwent DBE from December 2006 to July 2010. DBE was retrograde in 36 procedures, antegrade in six, and from both ends in five.

Anti-cancer effects of p21WAF1/CIP1 transcriptional activation induced by dsRNAs in human hepatocellular carcinoma cell lines.

Aim: To investigate the anti-cancer effects of p21WAF1/CIP1 transcriptional activation induced by dsRNAs in hepatocellular carcinoma (HCC) cell lines.

Methods: HCC cell lines BEL7402, SMMC-7721, MHCC97L, MHCC97H, and MHCCLM3 were used. HCC cells were treated with dsP21-322 (50 nmol/L), dsControl (50 nmol/L), siP21 (50 nmol/L), or mock transfection.

[Expression of zinc finger protein A20 in pediatric inflammatory bowel disease].

Objective: It is demonstrated that excessive activation of NF-κB is central to the pathogenesis of inflammatory bowel disease (IBD). Zinc finger protein A20 (A20) is a key player in the negative feedback regulation of NF-κB signaling in response to multiple stimuli and has been described as central gatekeeper in inflammation and immunity. Mice genetically deficient in A20 develop severe intestinal inflammation and have increased susceptibility to dextran sodium sulfate (DSS)-induced colitis.

Treatment of pediatric refractory Crohn's disease with thalidomide.

Aim: To assess the efficacy and tolerability of thalidomide in pediatric Crohn's disease (CD).

Methods: Six patients with refractory CD received thalidomide at an initial dose of 2 mg/kg per day for one month, then increased to 3 mg/kg per day or decreased to 1 mg/kg per day, and again further reduced to 0.5 mg/kg per day, according to the individual patient's response to the drug.