Salmonella Typhimurium screen identifies shifts in mixed-acid fermentation during gut colonization.

How enteric pathogens adapt their metabolism to a dynamic gut environment is not yet fully understood. To investigate how Salmonella enterica Typhimurium (S.Tm) colonizes the gut, we conducted an in vivo transposon mutagenesis screen in a gnotobiotic mouse model.

[Translated article] Pharmaceutical recommendations for therapeutic appropriateness in patients with multiple sclerosis.

Objective: To determine the prevalence of PIMDINAC criteria and to implement pharmacological interventions in a population with multiple sclerosis over 55 years of age.

Methods: Retrospective, observational, open-label study, including patients with multiple sclerosis aged 55 years and older during December 2022 and February 2023. The main variable determined was the percentage of compliance with the PIMDINAC criteria.

Identifying clinical response to daratumumab therapy in relapsed/refractory multiple myeloma using a patient-derived in vitro model.

Response to daratumumab in patients with relapsed/refractory multiple myeloma is heterogeneous, and a reliable biomarker of response is lacking. We aimed to develop a method that identifies response to daratumumab therapy. Patient-derived MM cells were collected before start of daratumumab treatment and were cultured in a hydrogel-based culture system.

Advancing Nutrition and Dietetics Research in Global Food and Nutrition Security: A Roundtable Meeting Report.

Food and nutrition security remains a relevant issue globally, impacting nutritional status and other health outcomes. This is further complicated by various environmental factors that impact stable access to, availability of, and utilization of nutritious foods. Nutrition and dietetics practitioners play an important role in the identification and treatment of food and nutrition security and are also well positioned to advance research that can support food and nutrition security solutions.

Dinaciclib synergizes with BH3 mimetics targeting BCL-2 and BCL-X in multiple myeloma cell lines partially dependent on MCL-1 and in plasma cells from patients.

A better understanding of multiple myeloma (MM) biology has led to the development of novel therapies. However, MM is still an incurable disease and new pharmacological strategies are needed. Dinaciclib, a multiple cyclin-dependent kinase (CDK) inhibitor, which inhibits CDK1, 2, 5 and 9, displays significant antimyeloma activity as found in phase II clinical trials.

Efficacy of interactive manual dexterity training after stroke: a pilot single-blinded randomized controlled trial.

Objective: To compare the efficacy of Dextrain Manipulandum™ training of dexterity components such as force control and independent finger movements, to dose-matched conventional therapy (CT) post-stroke.

Methods: A prospective, single-blind, pilot randomized clinical trial was conducted. Chronic-phase post-stroke patients with mild-to-moderate dexterity impairment (Box and Block Test (BBT) > 1) received 12 sessions of Dextrain or CT.

ASE recirculation effects in pulsed frequency shifted feedback lasers at large frequency shifts.

An analysis of the different emission regimes (continuous wave, Q-switched, and different forms of modelocking) of a C-band Er:fiber frequency shifted feedback laser at large frequency shifts is presented. We clarify the role of amplified spontaneous emission (ASE) recirculation in the origin of various spectral and dynamical properties of this type of laser. Specifically, we show that Q-switched pulses are supported by a noisy, quasiperiodic ASE recirculation pattern that univocally identifies the pulses within the sequence, and that these Q-switched pulses are chirped as a consequence of the frequency shift.

Novel Semiconductor Cu(CHNS)/ZnTiO/TiO for the Photoinactivation of and under Solar Light.

The use of semiconductors for bacterial photoinactivation is a promising approach that has attracted great interest in wastewater remediation. The photoinactivator Cu-TTC/ZTO/TO was synthesized by the solvothermal method from the coordination complex Cu(CHNS) (Cu-TTC) and the hybrid semiconductor ZnTiO/TiO (ZTO/TO). In this study, the effect of photocatalyst composition/concentration as well as radiation intensity on the photoinactivation of the gram-negative bacteria and the gram-positive bacteria in aqueous solutions was investigated.

Role of Guinea Pigs (Cavia porcellus) Raised as Livestock in Ecuadorian Andes as Reservoirs of Zoonotic Yeasts.

Guinea pigs (Cavia porcellus) have been reared for centuries in the Andean region for ceremonial purposes or as the main ingredient of traditional foods. The animals are kept in close proximity of households and interact closely with humans; this also occurs in western countries, where guinea pigs are considered pets. Even though it is acknowledged that domestic animals carry pathogenic yeasts in their tissues and organs that can cause human diseases, almost nothing is known in the case of guinea pigs.

Methodological considerations for ghrelin isoforms assay in clinical evaluation in anorexia nervosa.

The growing interest concerning the role of metabolic sensors in various eating disorders requires the implementation of a strict methodology to collect, store and process blood samples in clinical studies. In particular, measurement of isoforms of the appetite-stimulating hormone, ghrelin, has been challenging in clinical settings. Indeed the acyl ghrelin (AG) isoform is rapidly degraded into desacyl ghrelin (DAG) by blood esterases, thus optimal conditions for the conservation of AG and accurate determination of AG/DAG ratio should be used.

A novel tablet-based application for assessment of manual dexterity and its components: a reliability and validity study in healthy subjects.

Background: We developed five tablet-based tasks (applications) to measure multiple components of manual dexterity.

Aim: to test reliability and validity of tablet-based dexterity measures in healthy participants.

Methods: Tasks included: (1) Finger recognition to assess mental rotation capacity.

Shrinking of spatial hand representation but not of objects across the lifespan.

Perception and action are based on cerebral spatial representations of the body and the external world. However, spatial representations differ from the physical characteristics of body and external space (e.g.

Direct P70S6K1 inhibition to replace dexamethasone in synergistic combination with MCL-1 inhibition in multiple myeloma.

Novel combination therapies have markedly improved the lifespan of patients with multiple myeloma (MM), but drug resistance and disease relapse remain major clinical problems. Dexamethasone and other glucocorticoids are a cornerstone of conventional and new combination therapies for MM, although their use is accompanied by serious side effects. We aimed to uncover drug combinations that act in synergy and, as such, allow reduced dosing while remaining effective.

Advances and Perspectives in the Treatment of B-Cell Malignancies.

B-cell malignancies arise from different stages of B-cell differentiation and constitute a heterogeneous group of cancers including B-cell lymphomas, B-cell leukemias, and plasma cell dyscrasias [...

High throughput sequencing provides exact genomic locations of inducible prophages and accurate phage-to-host ratios in gut microbial strains.

Background: Temperate phages influence the density, diversity and function of bacterial populations. Historically, they have been described as carriers of toxins. More recently, they have also been recognised as direct modulators of the gut microbiome, and indirectly of host health and disease.

In Vitro Anti-Biofilm and Antibacterial Properties of sp. nov.

The aim of this study was to evaluate the potential anti-biofilm and antibacterial activities of sp. nov. To test anti-biofilm properties, and were grown in a biofilm model in the presence or not of sp.

An Integrative and Modular Framework to Recapitulate Emergent Behavior in Cell Migration.

Cell migration has been a subject of study in a broad variety of biological systems, from morphogenetic events during development to cancer progression. In this work, we describe single-cell movement in a modular framework from which we simulate the collective behavior of glioblastoma cells, the most prevalent and malignant primary brain tumor. We used the U87 cell line, which can be grown as a monolayer or spatially closely packed and organized in 3D structures called spheroids.

Regionalized tissue fluidization is required for epithelial gap closure during insect gastrulation.

Many animal embryos pull and close an epithelial sheet around the ellipsoidal egg surface during a gastrulation process known as epiboly. The ovoidal geometry dictates that the epithelial sheet first expands and subsequently compacts. Moreover, the spreading epithelium is mechanically stressed and this stress needs to be released.

Low prevalence of SARS-CoV-2 among patients presenting at a Parisian psychiatry University Hospital Group.

We examine the prevalence of SARS-CoV-2 infections among patients admitted to a Parisian psychiatric University Hospital Group (GHU). A total of 548 patients were admitted to the GHU..

Psychosocial Intervention in European Addictive Behaviour Recovery Programmes: A Qualitative Study.

Background: The vulnerability of drug users in treatment increases their risk of social exclusion. Psychosocial interventions aim to solve or minimise this risk, increasing social integration for individuals in treatment. In this study, the effectiveness of various European drug recovery programmes was studied by examining psychosocial intervention groups with vulnerable populations.