Publications by authors named "Cucco L"

Article Synopsis
  • Actinobacillus pleuropneumoniae causes porcine pleuropneumonia, a serious lung infection in pigs, and is typically managed using antibiotics like phenicols.
  • This study identifies three florfenicol-resistant isolates of A. pleuropneumoniae from Italian pig farms, all harboring the floR gene and showing varying susceptibility levels.
  • Whole genome sequencing revealed moderate virulence traits and the presence of a novel plasmid, pAp-floR, which is closely related to another plasmid and shows stability without selective pressure, emphasizing the need to monitor resistance in this bacterium.
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  • The study aimed to investigate the global spread of a specific linezolid-resistant Enterococcus faecalis strain (ST476), focusing on its genetic characteristics and distribution.
  • Researchers analyzed genomes from the NCBI database and found 66 isolates from 15 different countries, suggesting a wide geographical presence of this resistant strain primarily in humans and animals.
  • The findings indicate that the optrA gene, responsible for resistance, is closely associated with transposon Tn6674 and that the emergence of this clone poses significant public health risks, highlighting the need for a coordinated One Health strategy to manage its spread.
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Background: Streptococcus suis is an important pig pathogen and an emerging zoonotic agent. In a previous study, we described a high proportion of penicillin-resistant serotype 9 S. suis (SS9) isolates on pig farms in Italy.

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The spread of extended-spectrum β-lactamase (ESBL)-producing is a major public health issue. Bivalves are filter-feeder animals capable of bioaccumulating the microorganisms present in water. This physiological characteristic makes them both good indicators of environmental contamination and possible carriers of pathogenic bacteria, including those resistant to antimicrobials.

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Objectives: To investigate the optrA-carrying genetic elements and their transferability in two linezolid-resistant Streptococcus dysgalactiae subsp. equisimilis (SDSE) strains of swine origin.

Methods: SDSE strains (V220 and V1524) were phenotypically and genotypically characterized.

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Poultry is the most likely source of livestock-associated Extended Spectrum Beta-Lactamase (ESBL) and plasmid-mediated AmpC (pAmpC)-producing E. coli (EC) for humans. We tested the hypothesis that farming methods have an impact on the load of ESBL/pAmpC-EC in the gut of broilers at slaughter.

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Article Synopsis
  • * A study analyzed 78 isolates from diseased pigs in Italy, revealing that nearly 60% of infections were linked to serotypes 1/2 and 9, with distinct clustering patterns among different sequence types.
  • * Many serotype 9 isolates were found to be resistant to penicillin, raising concerns about treatment effectiveness and emphasizing the need for monitoring these S. suis strains in pigs for better disease management.
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  • Researchers studied 145 florfenicol-resistant enterococci from pig feces across 76 farms to identify linezolid resistance genes using PCR.
  • Out of 30 isolates with linezolid resistance genes, the most common was identified in 23 of them, while two other genes were less prevalent.
  • Whole Genome Sequencing (WGS) revealed variability in genetic contexts linked to transposons and plasmids, indicating that linezolid resistance genes can spread among enterococci in pigs, demonstrating a public health concern as some clones related to human isolates were also found.
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Contaminated pork is a significant source of foodborne Salmonellosis. Pork is contaminated at the slaughterhouse and the intestinal content is the predominant source of for carcass contamination. The prevalence of -positive pigs increases significantly when the time of transport to the slaughterhouse is longer than two hours.

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Objective: To analyse the diagnostic capacity of barium enema (BE) in the diagnostic investigation for Hirschsprung's disease (HD) was analyzed for transition zone (TZ) identification and rectosigmoid index (RSI) ≤1.0 determination.

Patients And Methods: BE images were analyzed retrospectively by 2 examiners and the results were compared with the histopathology of rectal biopsies.

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In rabbit, P. multocida is considered a predominant pathogenic agent; despite this, few data on the molecular epidemiology are available so far. The aim of this work was to characterize P.

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Pasteurella multocida is a widespread pathogen associated with major animal diseases of economic significance. Despite this, little is known about the capsular types, virulence gene pattern, and antimicrobial susceptibility of isolates from hosts affected by different diseases, and no data are available in Italy. One hundred eighty six isolates of P.

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Contaminated pork is a significant source of foodborne Salmonella infections. Pork is contaminated at the slaughterhouse; however, the mechanisms driving Salmonella contamination of carcasses are still poorly understood. The aim of this study was to investigate whether the amount of Salmonella carried by slaughtered pigs in their guts has an influence on carcass contamination.

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Salmonella Typhimurium (S. Typhimurium) is responsible for foodborne zoonotic infections that, in humans, induce self-limiting gastroenteritis. The aim of this study was to evaluate whether the wild-type strain S.

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Yersinia pseudotuberculosis is a pathogen that infects both animals and humans worldwide. The epidemiology of infection caused by Y. pseudotuberculosis is poorly understood; however, its outbreaks have been traced back to a probable source in wildlife.

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The aim of this study is to describe the prevalence and risk factors of Clostridium difficile shedding in six farms belonging to two companies in Northern Italy. Four hundred and twenty veal calves, randomly selected and individually identified, were sampled three times: at 0-16, 90-120, and 150 days after introduction. C.

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Atypical Yersinia pseudotuberculosis serotype O:3 was isolated from rectal contents of two wild boars hunted in Italy within a regional wildlife management program. No outbreak of yersiniosis was reported in this area in the same period and no lesions were found by the veterinarian at post-mortem inspection. Nevertheless, after histological examination, granulomatous lesions were detected in submandibular lymph nodes of one of the two wild boars.

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Aims: To estimate the efficacy of a self-monitoring-based disease management strategy in patients with Type 2 diabetes treated with oral agent monotherapy.

Methods: This was an open-label, randomized, pilot study, primarily led by diabetes nurses. Patients were randomly allocated to either a self-monitoring-based disease management strategy or usual care (ratio 3:1) and followed up for 6 months.

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The aim of the present study was to investigate the contamination sources and the transmission of Salmonella within a pig finishing herd in Italy. Nine sets of samples were collected during the fattening period from cleaned and disinfected pens, animals at different ages (4 days after arrival, 90, 150, 170 and 240 days of age) and at slaughter. Salmonella was isolated from cleaned pens, individual faecal samples, the truck used to transport the pigs to the abattoir and after slaughter (cecal contents, mesenteric lymph nodes and carcasses).

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