Sequencing analysis of forty-eight human image cDNA clones similar to Drosophila mutant protein.

We have sequenced 48 human IMAGE cDNA clones selected from the public EST database (dbEST) for their significant homology to Drosophila mutant genes. A dynamically updated analysis report was produced by BlastX and BlastN analysis searches in the latest databases available. This analysis led us to estimate the grade of similarity with homologous genes isolated in other species.

Gut lavage IgG and interleukin 1 receptor antagonist:interleukin 1 beta ratio as markers of intestinal inflammation in children with inflammatory bowel disease.

Background: Whole gut lavage is currently used as preparation before radiological or endoscopic examination of the large bowel.

Aim: To validate the gut lavage technique for the assessment of mucosal inflammation, by measuring intestinal IgG and interleukin 1 beta (IL-1 beta) in the fluid obtained.

Patients: Sixteen children with Crohn's disease (CD), 14 with ulcerative colitis (UC), and 22 age matched controls.

Cellobiose/mannitol sugar permeability test in children in relation to jejunal morphometry.

The ability of the sugar permeability test to detect minor degrees of mucosal damage is uncertain, particularly in children. This paper reviews experience with the cellobiose/mannitol test at a referral centre of paediatric gastroenterology, relating the results of intestinal permeability to the jejunal morphometry. Two hundred patients underwent the cellobiose/mannitol test at the same time as jejunal biopsy; morphometric analysis of the biopsy specimens was performed by a computerized image analysis system.