No evidence for the expression of renin-angiotensin-aldosterone system in otosclerotic stapes footplates.

Introduction: Recent studies have reported genetic associations between with single nucleotide polymorphism (SNP) of the several genes of the renin-angiotensin-aldosterone (RAA) system in otosclerosis without the confirmation of RAA system expression in human stapes footplates. There are conflicting results. These results are conflicting because RAA system expression has been attributed exclusively to neural, vascular, and renal tissues, exclusively.

Protein disorder prevails under crowded conditions.

Crowding caused by the high concentrations of macromolecules in the living cell changes chemical equilibria, thus promoting aggregation and folding reactions of proteins. The possible magnitude of this effect is particularly important with respect to the physiological structure of intrinsically disordered proteins (IDPs), which are devoid of well-defined three-dimensional structures in vitro. To probe this effect, we have studied the structural state of three IDPs, α-casein, MAP2c, and p21(Cip1), in the presence of the crowding agents Dextran and Ficoll 70 at concentrations up to 40%, and also the small-molecule osmolyte, trimethylamine N-oxide (TMAO), at concentrations up to 3.

Nontrivial spontaneous synchronization.

The collective behavior of an ensemble of multimode stochastic oscillators is investigated. The oscillators are pulse coupled; they are able to emit pulses and to detect the pulses emitted by the others. As a function of the output intensity in the system they can operate in different modes having different pulsing periods.