MS2 coliphage and E. coli UVB inactivation rates in optically clear water: dose, dose rate and temperature dependence.

Natural ultraviolet irradiance disinfection is known to play a significant role in both natural wastewater treatment systems and drinking water disinfection processes, while the influence of ultraviolet B (UVB) delivering method on sunlight disinfection outcome is still unclear. This study aims to determine the effects of environmentally relevant temperatures, UVB doses (J m) and dose rates (W m) on the inactivation and log reduction values (LRVs) of the F-RNA coliphage MS2 and Escherichia coli in optically clear water. E.

Comparison of the treatment performance of a high rate algal pond and a facultative waste stabilisation pond operating in rural South Australia.

South Australian community wastewater management schemes (CWMS) treat wastewater using waste stabilisation ponds before disposal or reuse. This study compared the performance of a facultative pond, 6,300 m, 27.5 d theoretical hydraulic retention time (THRT), with a high rate algal pond (HRAP) operated at depths of 0.

The influence of the microbial quality of wastewater, lettuce cultivars and enumeration technique when estimating the microbial contamination of wastewater-irrigated lettuce.

This study investigated the volume of wastewater retained on the surface of three different varieties of lettuce, Iceberg, Cos, and Oak leaf, following submersion in wastewater of different microbial qualities (10, 10, 10, and 10 E. coli MPN/100 mL) as a surrogate method for estimation of contamination of spray-irrigated lettuce. Uniquely, Escherichia coli was enumerated, after submersion, on both the outer and inner leaves and in a composite sample of lettuce.

Microbial risk in wastewater irrigated lettuce: comparing Escherichia coli contamination from an experimental site with a laboratory approach.

This study assessed the contamination of Escherichia coli, in lettuce grown with treated domestic wastewater in four different irrigation configurations: open spray, spray under plastic sheet cover, open drip and drip under plastic sheet cover. Samples of lettuce from each irrigation configuration and irrigating wastewater were collected during the growing season. No E.

Effective drinking water collaborations are not accidental: interagency relationships in the international water utility sector.

The role that deficient institutional relationships have played in aggravating drinking water incidents over the last 30 years has been identified in several inquiries of high profile drinking water safety events, peer-reviewed articles and media reports. These indicate that collaboration between water utilities and public health agencies (PHAs) during normal operations, and in emergencies, needs improvement. Here, critical elements of these interagency collaborations, that can be integrated within the corporate risk management structures of water utilities and PHAs alike, were identified using a grounded theory approach and 51 semi-structured interviews with utility and PHA staff.

Structure of nitrifying biofilms in a high-rate trickling filter designed for potable water pre-treatment.

This study examined the composition and structure of nitrifying biofilms sampled from a high-rate nitrifying trickling filter which was designed to pre-treat raw surface water for potable supply. The filter was operated under a range of feed water ammonia and organic carbon concentrations that mimicked the raw water quality of poorly protected catchments. The biofilm structure was examined using a combination of fluorescence in situ hybridisation and scanning electron microscopy.

Application of high rate nitrifying trickling filters to remove low concentrations of ammonia from reclaimed municipal wastewater.

The interference of ammonia with the chlorination process is a problem for many reclaimed water treatment plant operators. This paper presents the findings from a series of pilot experiments that investigated the efficacy of high flow rate nitrifying trickling filters (NTFs) for the removal of low concentrations of ammonia (0.5-3.

The impact of organic carbon on the performance of a high rate nitrifying trickling filter designed to pre-treat potable water.

The application of nitrifying trickling filters (NTFs) to potable water treatment is less well understood than their application to wastewater treatment, particularly regarding the effect of low ammonia substrate concentrations and organic carbon loading on filter performance. A large pilot-scale NTF was operated under conditions that simulated the raw water quality of poorly protected catchments typically found in SE Asia, with the objective of reducing the ammonia driven chlorine demand during disinfection. The efficacy of a high rate NTF to remove low concentrations of ammonia (0.

A review of the factors affecting sunlight inactivation of micro-organisms in waste stabilisation ponds: preliminary results for enterococci.

Waste stabilisation ponds (WSP) are efficient, cost-effective methods of treating wastewater in rural and remote communities in Australia. It is recognised that sunlight plays a significant role in their disinfection, however, due to the poor penetration of light in turbid waters it has been hypothesised that other mechanisms may also contribute to disinfection in WSPs. To date, studies have reported various and conflicting results with regards to the relative contributions of UVA, UVB, PAR and environmental factors including pH, DO and photo-sensitisers on micro-organism disinfection.

Nitrification potential in waste stabilisation ponds: comparison of a secondary and tertiary pond system.

This study compared the nitrification potential of two separate Waste Stabilisation Ponds (WSPs) operating under differing physical and chemical conditions. In order to probe the nitrification potential of each system, the oxidation of ammonium and also the intermediate product nitrite was assessed using both in situ and laboratory micro-scale incubations. The role of sediment in determining the nitrification potential of the two WSPs was also investigated.

Microbially influenced corrosion of galvanized steel pipes in aerobic water systems.

Aims: To investigate the role of heterotrophic bacteria in the corrosion of galvanized steel in the presence of water.

Methods And Results: Samples were taken from corroding galvanized steel pipes conveying water for specialist applications, and heterotrophic bacteria were isolated and cultured. The majority of bacteria were Gram-negative aerobes and included Pseudomonas sp.

Safe drinking water: critical components of effective inter-agency relationships.

The paper supports the development of evidence-based emergency management frameworks of cooperation between agencies in the area of drinking water and public health, as part of developing the overall risk management culture within water utilities. We employed a qualitative research design to understand critical gaps in inter-agency relations that aggravated past drinking water and health incidents and from these identified determinants of effective relationships. We identified six critical institutional relationship components that were deficient in past incidents, namely proactivity, communication, training, sharing expertise, trust and regulation.

Application of high rate nitrifying trickling filters for potable water treatment.

The interference of ammonia with chlorination is a prevalent problem encountered by water treatment plants located throughout South East Asia. The efficacy of high rate, plastic-packed trickling filters as a pre-treatment process to remove low concentrations of ammonia from polluted surface water was investigated. This paper presents the findings from a series of pilot experiments, which were designed to investigate the effect of specific conditions-namely low ammonia feed concentrations (0.

Relative performance of duckweed ponds and rock filtration as advanced in-pond wastewater treatment processes for upgrading waste stabilisation pond effluent: a pilot study.

An experimental pilot plant was operated over a five month period to assess the relative treatment performances of: a duckweed (DW) pond; a rock filter (RF); and an open pond (OP); for the upgrading of final WSP effluent prior to reuse applications. Each pilot treatment system consisted of three identical ponds arranged in three parallel series, each fed a continuous flow of wastewater from the local Bolivar treatment plant. Light penetration profiling for the DW and OP systems revealed some 55% greater light attenuation capacity for DW ponds compared to the OP system.

Temporal and spatial variation of physical, biological, and chemical parameters in a large waste stabilisation pond, and the implications for WSP modelling.

The spatial and temporal variation of physical, chemical, and biological parameters was determined, in summer and winter, at nine sites in a large (112 ha) waste stabilisation pond (WSP) at the Bolivar Wastewater Treatment Plant. Each site was extensively sampled over the course of one day, with the nine sites sampled over successive days at exactly the same times of day, progressing in the direction of bulk flow through the pond. Analyses of covariance were used to test the independent impact of site and climate on the way in which the mean values and stratification gradient of the physical, chemical, and biological parameters varied diurnally at each site.

Profiling and modelling of thermal changes in a large waste stabilisation pond.

A thermal profiling study was undertaken at four depths at each of nine sites, and at the inlets and outlets of a large waste stabilisation pond (WSP). Results were collected simultaneously using a network of 42 thermistors and dataloggers. Profiles at each site were categorised as either "stratified" or "unstratified", and persistence analysis was used to determine the frequency and persistence of stratification events at each of the nine sites.

Performance of a pilot-scale high rate algal pond system treating abattoir wastewater in rural South Australia: nitrification and denitrification.

As part of a study examining the efficacy of high-rate algal pond treatment of high-strength abattoir wastewater, the impact of pond configuration and loading rate on nitrification was determined. The extent of nitrification in all ponds was consistent with mass balance estimates of oxygen demand and availability. Deeper ponds were more stable nitrifying systems, with shallow ponds displaying greater variation in response to changes in nitrogen loading.

Changes in waste stabilisation pond performance resulting from the retrofit of activated sludge treatment upstream: part II--Management and operating issues.

Bolivar Wastewater Treatment Plant (WWTP) was originally commissioned with trickling filter secondary treatment, followed by waste stabilisation pond (WSP) treatment and marine discharge. In 1999, a dissolved air flotation/filtration (DAFF) plant was commissioned to treat a portion of the WSP effluent for horticultural reuse. In 2001, the trickling filters were replaced with activated sludge treatment.

Changes in waste stabilisation pond performance resulting from the retrofit of activated sludge treatment upstream: part I--water quality issues.

This paper describes changes in effluent quality occurring before and after an upgrade to the Bolivar Wastewater Treatment Plant in South Australia. Trickling filters (TF) were replaced with an activated sludge (AS) plant, prior to tertiary treatment using waste stabilisation ponds (WSPs). The water quality in the WSPs following the upgrade was significantly improved.

Use of microcosms to determine persistence of Escherichia coli in recreational coastal water and sediment and validation with in situ measurements.

Aims: To determine the persistence of the faecal indicator organism Escherichia coli in recreational coastal water and sediment using laboratory-based microcosms and validation with in situ measurements.

Methods And Results: Intact sediment cores were taken from three distinct coastal sites. Overlying estuarine water was inoculated with known concentrations of E.

The spatial significance of water quality indicators in waste stabilization ponds--limitations of residence time distribution analysis in predicting treatment efficiency.

Over the past fifty years, considerable research in waste stabilization pond operation has led to the development of a number of models used to describe the hydraulic regime and predict treatment efficiency. Models range in complexity from plug or completely mixed simplifications to computational fluid dynamics (CFD) models which are able to predict flow hydraulics at a local level. Information about the exit age of pond effluent can be used to estimate pollutant decay.

Characterisation of oxygen dynamics within a high-rate algal pond system used to treat abattoir wastewater.

As part of a study examining the efficacy of high-rate algal pond treatment of high-strength abattoir wastewater, the oxygen dynamics of a pilot scale system were characterised. The relationship between photosynthesis and irradiance was investigated using online data collected throughout a year of operation under varying conditions of climate and wastewater quality. Changes in climate were reflected in changes in the net rate of photosynthesis in deep ponds, consistent with adaptation of algal populations to changing light intensity, whilst the response of shallow ponds was more varied.

Use of image analysis to determine algal and bacterial biomass in a high rate algal pond following Percoll fractionation.

This paper reports the results of work to allow a more accurate enumeration, and gravimetric determination, of the relative proportions of algae and bacteria within the floccular matrix present in a High Rate Algal Pond (HRAP). Methodology involving a combination of physical and chemical treatments was used and the resulting material was separated using density gradient centrifugation. The "fractions" were analysed using microscopical image analysis.

Determination of faecal pollutants in Torrens and Patawalonga catchment waters in South Australia using faecal sterols.

This research investigated faecal pollution by sterol profiling in two Adelaide metropolitan catchments in winter 2000 and summer 2001. Water was sampled from five sites on the River Torrens and two sites on Patawalonga river catchment. The steroids detected were coprostanol, epicoprostanol, cholesterol, cholestanol, stigmasterol, betasitosterol, and stigmastanol.

Effectiveness of guideline faecal indicator organism values in estimation of exposure risk at recreational coastal sites.

Decay rates in coastal water and sediment for the bacterial pathogens Salmonella typhimurium and S. derby were compared in laboratory-based microcosms with results previously obtained for a number of faecal indicators. In general, the decay rates of Salmonella spp.