Highly Pathogenic Avian Influenza A(H5N1) from Wild Birds, Poultry, and Mammals, Peru.

We identified highly pathogenic avian influenza A(H5N1) virus clade 2.3.4.

Evidence of Oropouche Orthobunyavirus Infection, Colombia, 2017.

We describe an Oropouche orthobunyavirus infection in a women 28 years of age in Colombia. We confirmed the diagnosis by viral isolation, quantitative reverse transcription PCR, and phylogenetic analysis of the small, medium, and large genomic segments. The virus is related to a strain isolated in Ecuador in 2016.

Targeted full-genome amplification and sequencing of dengue virus types 1-4 from South America.

We report optimized workflows for full-genome sequencing of dengue viruses (DENVs) 1-4. Based on alignments of publicly available complete genomes we modified and expanded existing primers sets to amplify DENV genotypes that were previously difficult or impossible to sequence. We also report improvements to streamline laboratory handling, including a dual amplification strategy for easy and difficult to sequence "high-copy" and "low-copy" templates, respectively, and normalization of PCR cycling conditions across serotypes.

Molecular epidemiology of dengue virus type 3 in Northern South America: 2000--2005.

A phylogenetic approach was used to identify genetic variants of DENV-3 subtype III that may have emerged during or after its expansion throughout South America. We sequenced the capsid, premembrane/membrane and envelope genes from 22 DENV-3 strains isolated from Venezuela, Bolivia, Ecuador and Peru between 2000 and 2005. Phylogenetic analysis showed that the isolates sequenced in this study formed three clades within subtype III: one with the isolates from Venezuela, one with the Bolivian isolates and one with the isolates from Ecuador and Peru.