Fertility after insemination with frozen-thawed sperm using N-methylacetamide extender on the Combatiente Español avian breed.

The aim of this study was to evaluate the in vitro and in vivo quality of frozen-thawed sperm obtained from the Combatiente Español avian breed, with sperm having previously been diluted in N-methylacetamide (NMA). Experimental groups were established: fresh control semen (C); semen diluted without cryoprotectant (T1); semen diluted with extender containing NMA (T2); frozen-thawed sperm (with NMA) containing 500 × 10 spermatozoa (T3); frozen-thawed sperm (with NMA) containing 250 × 10 spermatozoa (T4). In the different groups, sperm motility and viability were assessed using a computer-assisted semen analyzer and flow cytometer, respectively.