Publications by authors named "Cristina Sanchez-Resalt"
Article Synopsis
- The study aimed to create and test a sensitive method for detecting IgG anti-SARS-CoV-2 antibodies in saliva samples as an alternative to serum testing.
- A two-step immunoassay was developed, and various factors, including saliva collection methods and protein content correction, were analyzed for their impact on detecting antibody levels after vaccination.
- Results indicated that while saliva had lower antibody concentrations than serum, it showed similar response patterns, suggesting the method is effective, though saliva may be less sensitive during early immune responses.
High ferritin serum levels can be found in patients with macrophage activation syndrome, and increased serum ferritin due to cytokine storm have been reported in severe COVID-19 patients. Saliva is being increasingly used in COVID-19 tests as a diagnostic sample for virus detection and quantification. This study aimed to evaluate the possible changes in ferritin in saliva in COVID-19 patients.
View Article and Find Full Text PDFObjectives: To evaluate four sample treatments in a safe and straightforward procedure to detect SARS-CoV-2 in saliva.
Methods: Four sample treatments were evaluated in a 3-step procedure to detect SARS-CoV-2 in saliva: 1) heating at 95 °C for 5 min for sample inactivation; 2) sample treatment; 3) analysis by reverse-transcription loop-mediated isothermal amplification (LAMP). Saliva samples used were from infected individuals or were spiked with known quantities of viral particles.
Objectives: The aim of the present study was to validate a commercially available automated assay for the measurement of total adenosine deaminase (tADA) and its isoenzymes (ADA1 and ADA2) in saliva in a fast and accurate way, and evaluate the possible changes of these analytes in individuals with SARS-CoV-2 infection.
Methods: The validation, in addition to the evaluation of precision and accuracy, included the analysis of the effects of the main procedures that are currently being used for SARS-CoV-2 inactivation in saliva and a pilot study to evaluate the possible changes in salivary tADA and isoenzymes in individuals infected with SARS-CoV-2.
Results: The automated assay proved to be accurate and precise, with intra- and inter-assay coefficients of variation below 8.
Background: The aim of this study was to evaluate changes in the enzymes creatine kinase (CK), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) in saliva before and after an intense exercise consisting of a futsal match.
Methods: CK, LDH and AST were analyzed in saliva and serum samples of eleven, injury-free, amateur young men before and 30 minutes, 12 hours and 36 hours after a futsal match.
Results: A significant increase in CK, LDH and AST was observed after the game in serum samples.